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第一作者:Huayue Sun
第一单位:中国农业大学
通讯作者:Weiwei Jin
Abstract
背景回顾1:The floral transition of the maize (Zea mays ssp. mays ) shoot apical meristem determines leaf number and flowering time, which are key traits influencing local adaptation and yield potential.
背景回顾2:dlf1 (delayed flowering1) encodes a basic leucine zipper protein that interacts with the florigen ZCN8 to mediate floral induction in the shoot apex.
提出问题:However, the mechanism of how dlf1 promotes floral transition remains largely unknown.
QTL:We demonstrate that dlf1 underlies qLB7‐1, a quantitative trait locus controlling leaf number and flowering time that was identified in a BC2S3 population derived from a cross between maize and its wild ancestor, teosinte (Zea mays ssp. parviglumis ).
组学:Transcriptome sequencing and chromatin immunoprecipitation sequencing demonstrated that DLF1 binds the core promoter of two AP1 /FUL subfamily MADS‐box genes, ZmMADS4 and ZmMADS67 , to activate their expression.
试验:Knocking out ZmMADS4 and ZmMADS67 both increased leaf number and delayed flowering, indicating that they promote the floral transition.
选择与适应:Nucleotide diversity analysis revealed that dlf1 and ZmMADS67 were targeted by selection, suggesting that they may have played important roles in maize flowering time adaptation.
结论:We show that dlf1 promotes maize floral transition by directly activating ZmMADS4 and ZmMADS67 in the shoot apex, providing novel insights into the mechanism of maize floral transition.
摘 要
玉米的成花转变中,茎尖分生组织决定了叶片数量以及开花时间,这对于玉米的地域适应以及产量都是关键性状。迟花突变dlf1编码一个亮氨酸拉链蛋白,会与开花素ZCN8相互作用,介导茎尖中的成花转变。然而,有关dlf1如何促进成花转变的分子机制还不清楚。本文中,作者发现dlf1突变位于数量性状位点qLB7‐1内,该位点是基于玉米与其野生祖先种大刍草杂交构建的一个BC2S3群体所鉴定出来的,与叶片数量及开花时间相关的QTL。转录组测序和染色质免疫沉淀测序的结果显示DLF1会结合到AP1/FUL亚家族中两个MADS‐box基因ZmMADS4和ZmMADS67的核心启动子区,来激活这两个基因的表达。敲除ZmMADS4和ZmMADS67均会增加叶片数量和延迟开花,说明这两个基因促进了成花转变。核苷酸多态性分析显示dlf1和ZmMADS67基因受到了选择,说明其可能在玉米开始时间适应过程中发挥了非常重要的作用。本文的研究揭示了dlf1在茎尖通过直接激活ZmMADS4和ZmMADS67基因的表达来促进玉米的成花转变,为玉米的成花转变分子调控机制提供了新的视野。
通讯作者
**金危危**
个人简介:
1993年,湖南农业大学,学士;
2001年,武汉大学,博士;
2002-2004年,美国威斯康星大学,博后。
研究方向:
玉米等作物染色体着丝粒的结构和功能研究;
玉米近缘种(薏苡、大刍草等)的基因组学研究;
玉米单倍体诱导机理的研究;
玉米株型、减数分裂相关基因及其他重要功能基因的克隆;
其他植物分子细胞遗传学研究。
doi: 10.1111/nph.16772
Journal: New Phytologist
Published date: June 24, 2020
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