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The Human Protein Atlas
程琳 2022-6-11 03:25
The Human Protein Atlas (https://www.proteinatlas.org/) is a Swedish-based program started in 2003 with the aim to map all the human proteins in cells, tissues and organs using integration of various omics technologies, including antibody-based imaging, mass spectrometry-based proteomics, transcri ...
1484 次阅读|没有评论
Annexin A1和Annexin V的区别
程琳 2022-5-18 12:26
Annexin A1: may have potential anti-inflammatory activity. Annexin V: apoptotic marker, identify the integrity of cell membrane (used for FC) Annexin A1 belongs to the annexin family of Ca 2+ -dependent phospholipid -binding proteins that have a mole ...
1197 次阅读|1 个评论
human embryos are developing 3 times slower than the mice
程琳 2022-4-23 12:14
如题,human embryos are developing 3 times slower than the mice。这是我前年参加ISSCR会议得到的一个结论。供自己参考。
1305 次阅读|没有评论
Frequently asked questions about the cell dyes
程琳 2022-1-15 04:26
How to determine the optimum concentration of dye The best concentration for Calcein-AM and PI depends on the cell type, so it is necessary to determine the concentration when staining each cell. The best concentration can be determined using the following protocol. Optimum concentration for P ...
767 次阅读|没有评论
How long are retinal ganglion cells?
程琳 2021-12-24 08:36
~50 mm Retinal ganglion cells (RGCs) are specialized projection neurons that relay an immense amount of visual information from the retina to the brain. RGC signal inputs are collected by dendrites and output is distributed from the cell body via very thin (0.5-1 μm) and long (~50 m ...
767 次阅读|没有评论
when to use "SD" and "SEM"?
程琳 2021-12-10 05:27
The standard deviation , or SD , measures the amount of variability or dispersion for a subject set of data from the mean, while the standard error of the mean, or SEM, measures how far the sample mean of the data is likely to be from the true population mean. The SEM is al ...
1179 次阅读|没有评论
[转载]细胞出现空泡化是原因 如何解决方案
程琳 2021-12-4 00:22
细胞是试验的基础,好的细胞是成功的开始,然而在养细胞的时候,我们总是遇到各种各样的问题,这里说下细胞出现空泡化的原因及解决方案! 细胞出现空泡化 1 )细胞老化 原代细胞培养的过程中经常出现这样的情况,是有些细胞处于终末分化状态,不会再分裂,时间长了就会空泡化而死亡。 传 ...
3126 次阅读|没有评论
Cell Counting Kit-8 (CCK8)介绍
程琳 2021-9-9 04:43
Background: Determining the cell # is based on various cell functions, such as enzyme activity, cell membrane permeability, cell adherence, ATP production, co-enzyme production, and nucleotide uptake activity. A variety of methods such as Colony Formation , Crystal Violet , Tritium-Labeled Thymi ...
3376 次阅读|1 个评论
美天旎磁珠知识点介绍
程琳 2021-9-8 23:42
MACS磁珠:或者称为“微珠”,是一种高度特异性monoclonial Antibody相偶联的超顺磁化微粒,可用于目的细胞的磁性标记。 50nm大小 无毒 可生物降解 不影响细胞特性或活性 磁珠无需解离,可以直接进行FC或cell culture 磁性标记只用20%-30%的结合位点,不影响后续使用细胞进行荧光抗体标记 磁珠孵育 ...
4623 次阅读|没有评论
Rosa26基因介绍
程琳 2021-8-28 01:06
Rosa26 is a genomic mouse locus commonly used to knock-in cDNA constructs for ubiquitous or conditional gene expression in transgenic mice. The human homolog of the ROSA26 locus has been identified.ROSA stands for R everse O rientation S plice A ccepto ...
6911 次阅读|没有评论

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