http://en.wikipedia.org/wiki/Restriction_site_associated_DNA_markers

http://ngs-expert.com/tag/rad-seq/

https://www.wiki.ed.ac.uk/display/RADSequencing/Home

http://bbs.sciencenet.cn/home.php?mod=space&uid=491809&do=blog&id=444728

http://datadryad.org/resource/doi:10.5061/dryad.1mj31/1

酶切位点相关DNA 测序(restriction site-associated DNA sequencing, RAD-Seq)技术，它是指对全基因组中限制性内切酶，酶切位点附近的片段进行测序的方法,类似的还有GBS (Genotyping-by-Sequencing) 。RAD-Seq提供了一个开发SNPs 极为有效的方法,其优点是节省传统测序的试验成本，能够快速准确的定位出数以千计的基因标记，且已广泛的应用于模式生物和非模式生物的相关研究领域中。获得的大量SNP分型信息后可以用于遗传连锁图谱的构建，QTL定位，群体遗传分析，谱系地理分析和系统发育分析等。

目前RAD-Seq的数据分析软件主要是有Stacks（http://creskolab.uoregon.edu/stacks/）和PyRAD（http://code.google.com/p/pyrad/）。

参考文献：

我们已发的文章，关于系统发育的：

Wang  XQ, Zhao L, Eaton DA, Li DZ, Guo ZH (2013) Identification of SNP  markers for inferring phylogeny in temperate bamboos (Poaceae:  Bambusoideae) using RAD sequencing. Molecular Ecology  Resources,13(5),938–945.

其他文献：

Eaton DA, Ree RH (2013) Inferring Phylogeny and Introgression using RADseq Data: An Example from Flowering Plants (Pedicularis: Orobanchaceae). Syst Biol.

Cariou,  M., Duret, L., and Charlat, S. (2013). Is RAD-seq suitable for  phylogenetic inference? an in silico assessment and optimization.  Ecology and evolution,3(4),846–852.

Catchen JM, Amores A, Hohenlohe P, Cresko W, Postlethwait JH (2011) Stacks: building and genotyping Loci de novo from short-read sequences. G3 (Bethesda) 1: 171-182.

Emerson KJ, Merz CR, Catchen JM, Hohenlohe PA, Cresko WA, et al. (2010) Resolving postglacial phylogeography using high-throughput sequencing. Proc Natl Acad Sci U S A 107: 16196-16200.

Miller MR, Dunham JP, Amores A, Cresko WA, Johnson EA (2007) Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers. Genome Res 17: 240-248.