▲ Vol 43 Issue 03 | 15 October , 2025

西太平洋海山区浮游动物优势物种名录

孙柔鑫  ,  王彦国 ,  杨燕燕 ,  陈杨航 ,  项鹏 ,  王春光 ,  邢炳鹏

西太平洋海域具有高物种多样性的特点。西太平洋海山区具有独特的地理形态,在该区开展浮游动物种类多样性研究重要且必要。本研究利用标准大型浮游生物网采集了西太平洋海山附近8个站位的浮游动物样品,通过镜检鉴定分析出15个浮游动物优势种,包括粗乳点水蚤Pleuromamma robusta、小哲水蚤Nannocalanus minor、长角海羽水蚤Haloptilus longicornis、美丽大眼水蚤Corycaeus speciosus、丹氏厚壳水蚤Scolecithrix danae、窄缝真刺水蚤Euchaeta rimana、爪室水母Chelophyes appendiculata、巴斯水母Bassia bassensis、尖角水母Eudoxoides mitra、小拟多面水母Abylopsis eschscholtzi、方拟多面水母Abylopsis tetragona、肥胖箭虫 Sagitta enflata、太平洋箭虫Sagitta pacifica、长拟浮萤Paraconchoecia oblonga和萨利纽鳃樽Thalia democratica。利用显微拍摄获取了这些优势种的形态特征图,并附以形态特征描述及其地理分布和生态习性,为今后深入研究西太平洋海域浮游动物的种类组成、分布、起源、扩散、区系特征和地理学特点提供了科学依据和参考。

http://hyxyj.sio.org.cn/CN/10.3969/j.issn.1001-909X.2025.03.010

▲ Vol 25 Issue 20 | 21 October , 2025

A high-sensitivity and clogging-free microfluidic impedance flow cytometer based on three-dimensional hydrodynamic focusing

Xiao Chen, Tingxuan Fang, Yimin Li,Jie Zhang, Xiaoye Huo, Junbo Wang,  Xuzhen Qin,  Yueying Li, Yi Zhang and Jian Chen

Microfluidic impedance flow cytometry has functioned as an enabling instrument in single-cell analysis, which, however, suffers from the limiting tradeoff between high sensitivity and clogging-free operation. In order to address this issue, this study presented a microfluidic impedance flow cytometer based on three-dimensional (3D) hydrodynamic focusing, in which the crossflow of conductive sample fluids and insulating sheath fluids was leveraged to centralize and restrict electric field lines to the sample fluid, thereby achieving high impedance sensitivity of single cells without the concern of channel blockage. Different from conventional impedance flow cytometry, in this study, impedance amplitude dips (rather than pulse singles) generated by single microparticles traveling through the 3D hydrodynamic focusing region were experimentally validated using microbeads. Based on the home-developed microfluidic impedance flow cytometer, high-sensitivity and clogging-free impedance profiles of three leukemia cell lines (K562, Jurkat, and HL-60) and four types of purified leukocytes (neutrophil, eosinophil, monocyte, and lymphocyte) were quantified as −8.01 ± 2.96%, −4.53 ± 1.09%, −6.36 ± 1.54%; −8.11 ± 0.84%, −7.23 ± 1.06%, −9.05 ± 2.00% and −5.68 ± 1.24%, respectively. When a recurrent neural network was adopted for cell-type classification, high classification accuracies of 93.9% for three leukemia cell lines and 87.8% for four types of purified leukocytes were achieved. This study presented a promising impedance flow cytometer that combines high sensitivity with sustainable working capabilities, potentially overcoming the limitations of conventional microfluidic impedance flow cytometry and significantly advancing its commercial development.

https://pubs.rsc.org/en/content/articlelanding/2025/lc/d5lc00571j

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静远嘲风-南京（MY Scimage） 成立于2007年，嘲风取自中国传统文化中龙生九子，子子不同的传说，嘲风为守护屋脊之瑞兽，喜登高望远；静远取自成语“宁静致远”，登高莫忘初心，远观而不可务远。

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