这个小组前段时间刚发表了顺铂肾脏毒性的保护作用，采用血氧依赖的MRI技术研究，研究手段几乎没有什么区别，只是换了一下动物模型，本来我开始以为是同一个研究，结果没有注意，但仔细一看，原来是不同的研究。这个研究特别强调了一种避免氢气从饲养瓶泄露的方法，就是在金属管内放了两个小球。大家不要被这个东西给糊弄了，因为氢气在水中的溶解度只有2%，浓度的降低并不是通过瓶口的释放，而是在瓶子内释放到气垫中，如果希望减少氢气浓度的降低，唯一的办法是采用软质包装，避免气泡形成。否则的话是没有任何意义的。我想他们肯定没有进行浓度的对比检测，否则不会把这个内容写到文章中，

http://www.jstage.jst.go.jp/article/mrms/10/3/169/_pdf

Magn Reson Med Sci. 2011;10(3):169-76.

Protective Effect of Hydrogen-rich Water against Gentamicin-induced Nephrotoxicity in Rats using Blood Oxygenation Level-dependent MR Imaging.

Matsushita T, Kusakabe Y, Kitamura A, Okada S, Murase K.

Source

Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University.

Abstract

Purpose: We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective effect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging. Materials and Methods: We acquired T(2)*-weighted images (T(2)*WI) of 21 rats on Days 0, 2, 4, and 7 using a 1.5-tesla MR imaging system. The rats were divided into 3 groups of seven each: control rats had free access to standard water and no gentamicin (GM) injection; rats designated the GM group had free access to standard water and were injected with GM (80 mg/kg/day) subcutaneously for 7 days; and the third group, designated the GM+HW group, had free access to HW and were injected with GM. R(2)* (=1/T(2)*) was estimated from T(2)*WI. Results: R(2)* values in the cortex were significantly decreased on Days 2, 4, and 7 compared with those on Day 0 in the GM group but not significantly changed in the control and GM+HW groups. R(2)* values in the medulla did not change significantly in any group. Conclusions: Our findings suggested reduced oxygen utility, mainly in the cortex, in gentamicin-induced nephrotoxicity and an ameliorative effect of hydrogen-rich water against GIN.