对费用性肢体萎缩无效.pdf

最近来自日本的报道是关于氢气对瘫痪后肢体萎缩作用的研究。研究用大鼠做模型，造成下肢废用，模拟瘫痪后下肢萎缩，或者太空飞行性肢体萎缩（有军方单位的参与），动物分为四 组：正常对照，模型后分三组，正常水，电解水和氢气饱和的电解水。模型三周后，进行观察。研究指标有直接测定腓肠肌的重量，检测MDA和8OHdG(两个氧化指标)，SOD活性。虽然结果综合显示，氢气具有一定作用，但没有统计学差异。结论是氢气没有这方面的保护作用。但我认为这个研究存在一些问题。首先是采用的指标太粗糟，不能准确检测效应。至少应该有肌肉的蛋白含量分析，组织学改变，特殊蛋白水平的检测等。也许这方面的研究没有那么多指标。但匆忙得出这样的结论有点遗憾。例如腓肠肌的重量，氢气组的下降程度是7%，而损伤组的是14%。也就是相对减少了50%。不知道要达到多少才能算有效。而且所有的指标之间都具有相关性，就是各组的相对变化都符合氢气治疗有效的推断。当然，从统计学角度，氢气就是无效。也应该尊重这个判断。只不过我相信很快将有更准确的研究来确定这个效应。

J Physiol Anthropol. 2011;30(5):195-201.

Effect of molecular hydrogen saturated alkaline electrolyzed water on disuse muscle atrophy in gastrocnemius muscle.

Fujita R, Tanaka Y, Saihara Y, Yamakita M, Ando D, Koyama K.

Source

Graduate School of Medicine, Osaka University.

Abstract

The objectives of this paper were to determine the level of oxidative stress in atrophied gastrocnemius, and to verify the effect of molecular hydrogen (H(2)) saturated alkaline electrolyzed water (HSW) on gastrocnemius atrophy by modifying the redox status, indicated by 8-hydroxy-2'-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and superoxide dismutase (SOD)-like activity. Female Wistar rats were divided into four groups: (1) the control (CONT); (2) the Hindlimb unloading (HU, for 3 weeks) given purified normal water (HU-NW); (3) the HU given alkaline electrolyzed reduced water (HU-AEW); and (4) the HU given HSW (HU-HSW). We showed that 8-OHdG, but not MDA, significantly increased by 149% and 145% in HU-NW and HU-AEW, respectively, when compared with CONT. In contrast, there was a trend toward suppression in 8-OHdG levels (increased by 95% compared with CONT) by treatment of HSW, though this effect was not prominent. Additionally, SOD-like activity significantly increased in both HU-NW (184%) and HU-AEW (199%) when compared with CONT. This result suggests the elevation of O(2)(-·) in the atrophied gastrocnemius. However, upregulation of SOD-like activity in the HU-HSW was increased by only 169% compared with CONT, though this difference is too small to detect statistical significance. HU led to 13% and 15% reduction of gastrocnemius wet weights in HU-NW and HU-AEW, respectively, compared with CONT. And the reduction of gastrocnemius wet weights in HU-HSW was attenuated by 7% compared with CONT. The gastrocnemius wet weights in the HU-HSW group were significantly greater than those in the HU-AEW, but not statistically significant with HU-NW. These results indicate that HU causes an increase in oxidative stress, but, in this experimental protocol, continuous consumption of HSW during HU does not demonstrate successful attenuation of oxidative stress and HU-mediated gastrocnemius atrophy.

PMID:

21963827

[PubMed - in process]

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