aBIOTECH | 最新『基因编辑』主题精选

本期精选 aBIOTECH 『基因编辑』专刊最新上线论文，欢迎学者们下载、阅读、转发。『基因编辑』专刊将于近期正式出版，敬请期待！

1

Exploiting viral vectors to deliver genome editing reagents in plants

万建民/董小鸥团队综述植物病毒载体在基因编辑元件递送中的应用

Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong & Jianmin Wan 

Fig. 1 Using positive stranded RNA virus (A) and negative stranded RNA virus (B) to deliver gene editing elements

2

Efficient genome editing in rice with miniature Cas12f variants

 万建民/谭俊杰团队报道水稻超迷你CRISPR-Cas12f基因编辑工具

Zhengyan Ye, Yuanyan Zhang, Shiqi He, Shaokang Li, Longjiong Luo, Yanbiao Zhou, Junjie Tan & Jianmin Wan 

 Fig. 1 Super Mini CRISPR-Cas12f Effectively Implements Rice Genome Editing

3

Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations

陈其军团队优化LbCas12a变体使其在拟南芥基因组编辑中达到实用化程度

Cuiping Xin, Dexin Qiao, Junya Wang, Wei Sun, Zhenghong Cao, Yu Lu, Yuanyuan Jiang, Yiping Chai, Xue-Chen Wang & Qi-jun Chen 

Fig. 1 Comparison of Editing Efficiency of Five Cas12a Variants

4

Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants

夏兰琴团队在水稻中实现腺嘌呤单碱基转换和颠换

Yucai Li, Shaoya Li, Chenfei Li, Chen Zhang, Lei Yan, Jingying Li, Yubing He, Yan Guo & Lanqin Xia 

Fig. 1 Schematic diagram of plant rAKBEs carrier structure

5

A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

李剑峰团队开发富集多基因CRISPR突变体和剔除Cas9转基因的双功能筛选系统

Feng-Zhu Wang, Ying Bao, Zhenxiang Li, Xiangyu Xiong & Jian-Feng Li 

Fig. 1 D-amino acid oxidase TvDAO can be used for dual functional screening of transgenicArabidopsis plant

6

Developing a CRISPR/FrCas9 system for core promoter editing in rice

魏鹏程团队利用FrCas9系统编辑水稻基因核心启动子

Hui Wang, Jian Ding, Jingyan Zhu, Xiaoshuang Liu, Rongfang Xu, Ruiying Qin, Dongfang Gu, Min Li, Pengcheng Wei & Juan Li 

 Fig. 1 Rice FrCas9 gene editing tool and its utilization

7

Genome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield

关跃峰团队创制低植酸与产量表型平衡的基因编辑大豆

Wenxin Lin, Mengyan Bai, Chunyan Peng, Huaqin Kuang, Fanjiang Kong & Yuefeng Guan 

Fig. 1 Creation of phytic acid mutants and analysis of agronomic traits

8

Expanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in Brassica

赵建军/李君团队扩展芸薹属蔬菜中CRISPR/Cas9基因编辑靶向范围

Wenjing Li, Xuan Li, Chunyang Wang, Guanzhong Huo, Xinru Zhang, Jintai Yu, Xiaoxiao Yu, Jing Li, Chao Zhang, Jianjun Zhao, Yan Li & Jun Li 

 Fig. 1 Expanding the gene editing range of Chinese cabbage and cabbage using Cas9-NG, SpG, and SpRY variants

9

The RUBY reporter for visual selection in soybean genome editing

侯文胜团队建立基因编辑大豆可视化识别技术体系

Li Chen, Yupeng Cai, Xiaoqian Liu, Weiwei Yao, Shuiqing Wu & Wensheng Hou 

Fig. 1 RUBY helps to achieve visual screening of genetically modified soybean gene edited plants

10

Efficient and precise genomic deletion in rice using enhanced prime editing

任雯/杨进孝/赵久然团队利用增强型引导编辑技术实现高效精确的基因组片段缺失

Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao & Jinxiao Yang 

Fig. 1 Implementing precise genome deletion in rice using PE3, PDel, and PDel/Syn strategies

11

C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases

谢卡斌团队通过引入C末端移码突变获得可育和可遗传的MAPK/RLK基因敲除突变体

Yun Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen & Kabin Xie 

Fig. 1 Targeted editing of C-terminal sequences of OsMPK1, WAK R942, LRR-RLK R753

12

Regulation of gene-edited plants in Europe: from the valley of tears into the shining sun？

欧盟新基因（组）技术监管政策的演变

Holger Puchta 

13

Integrating machine learning and genome editing for crop improvement

张韬-机器学习方法在作物基因编辑中的应用

Long Chen, Guanqing Liu & Tao Zhang 

Fig. 1 The Application of Machine Learning in Gene Editing

14

Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

Debasmita Panda, Subhasis Karmakar, Manaswini Dash, Swagat Kumar Tripathy, Priya Das, Sagar Banerjee, Yiping Qi, Sanghamitra Samantaray, Pradipta Kumar Mohapatra, Mirza J. Baig & Kutubuddin A. Molla

Fig. 1 Viability of isolated protoplasts from rice and Arabidopsis assessed by Evan’s blue staining

15

Consumer transparency in the production chain for plant varieties produced using new genomic techniques

J. M. Lukasiewicz, C. C. M. van de Wiel, L. A. P. Lotz & M. J. M. Smulders