Temporal metabolite responsiveness of microbiota in the tea plant phyllosphere promotes continuous suppression of fungal pathogens

背景介绍：A broad spectrum of rhizosphere bacteria and fungi were shown to play a central role for health, fitness and productivity of their host plants. 

广泛的根际细菌和真菌被证明对寄主植物的健康、适合性和生产力起着核心作用。

提出问题#1：However, implications of host metabolism on microbiota assembly in the phyllosphere and potential consequences for holobiont functioning were sparsely addressed. 

#2：Previous observations indicated that tea plants might reduce disease occurrence in various forests located in their proximity; the underlying mechanisms and potential implications of the phyllosphere microbiota remained elusive.

然而，寄主新陈代谢对叶圈内微生物区系组装的影响以及对全息菌功能的潜在影响却鲜有涉及。

以前的观察表明，茶树可能会减少其附近各种森林中的病害发生；叶圈微生物区系的潜在机制和潜在影响仍然难以捉摸。

研究目的：This study aimed at deciphering microbiome assembly in the tea plant phyllosphere throughout shoot development as well as elucidating potential implications of host metabolites in this process. The main focus was to explore hidden interconnections between the homeostasis of the phyllosphere microbiome and resistance to fungal pathogens.

本研究旨在破译茶树枝条发育过程中叶圈内的微生物群组装，并阐明寄主代谢产物在这一过程中的潜在意义。主要的焦点是探索叶圈微生物群的动态平衡和对真菌病原体的抗性之间隐藏的相互联系。

研究内容：Profiling of host metabolites and microbiome analyses based on high-throughput sequencing were integrated to identify drivers of microbiome assembly throughout shoot development in the phyllosphere of tea plants. This was complemented by tracking of beneficial microorganisms in all compartments of the plant. Synthetic assemblages (SynAss), bioassays and field surveys were implemented to verify functioning of the phyllosphere microbiota.

结合寄主代谢物图谱和基于高通量测序的微生物群分析，以确定茶树枝条发育过程中微生物群组装的驱动因素。此外，还对植物所有隔间中的有益微生物进行了跟踪。采用合成集合体(SynAss)、生物测定和野外调查来验证叶圈微生物区系的功能。

研究结果#1：Theophylline and epigallocatechin gallate, two prevalent metabolites at the early and late shoot development stage respectively, were identified as the main drivers of microbial community assembly. Flavobacterium and Myriangium were distinct microbial responders at the early stage, while Parabacteroides and Mortierella were more enriched at the late stage. 

#2：Reconstructed, stage-specific SynAss suppressed various tree phytopathogens by 13.0%-69.3% in vitro and reduced disease incidence by 8.24%-41.3% in vivo.

茶碱和表没食子儿茶素没食子酸酯分别是地上部发育早期和后期的两种主要代谢物，它们是微生物群落聚集的主要驱动力。黄杆菌和Myriangium是早期明显的微生物响应菌，而副杆菌和被孢霉则在后期表现出更强的富集性。重组的阶段特异性SynAss在体外对各种树木病原菌的抑制率为13.0%~69.3%，在体内的发病率为8.24%~41.3%。

结论：The findings indicate that a functional phyllosphere microbiota was assembled along with development-specific metabolites in tea plants, which continuously suppressed prevalent funga.

这些发现表明，茶树中的功能叶圈微生物群与发育特异的代谢产物一起组装在一起，不断抑制流行的真菌病原菌。对茶树微生物区系的时间分辨代谢物响应的洞察可以为病害管理提供新的解决方案。

原文链接：https://www.sciencedirect.com/science/article/pii/S2090123221001995

作者简介：

**王蒙岑

利用化学生态学、遗传学、微生物组学、分子生物学等研究手段和技术体系，揭示水稻-微生物-环境互作的分子机制，研发水稻病害和稻米生物毒素污染的新型防控技术 (ORCID主页 https://orcid.org/0000-0001-7169-6779)。

工作学习经历

2021年12月-至今              浙江大学农业与生物技术学院，教授，博士生导师

2018年12月-2021年12月  浙江大学农业与生物技术学院，副教授，博士生导师

2013年10月-2018年12月  浙江大学农业与生物技术学院，讲师，硕士生导师

2010年10月-2013年09月  日本北海道大学农学院，博士（CSC公派攻博，应用生物科学专攻，生态化学生物学）

2007年09月-2010年03月  浙江大学农业与生物技术学院，硕士（植物保护，农药学）

2003年09月-2007年06月  湖南农业大学植物保护学院，学士（植物保护，植物病理学）

Journal: Journal of Advanced Research

Published date: October 18, 2021