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第一作者:Eun Seon Lee
第一单位:韩国庆尚国立大学
第一通讯:Sang Yeol Lee
Abstract
背景1-CBFs:The activities of cold-responsive C-repeat-binding transcription factors (CBFs) are tightly controlled as they not only induce cold tolerance but also regulate normal plant growth under temperate conditions. 背景2-Trx-h2:Thioredoxin h2 (Trx-h2)—a cytosolic redox protein identified as an interacting partner of CBF1—is normally anchored to cytoplasmic endomembranes through myristoylation at the second glycine residue. 结果1-低温诱导Trx-h2进核,减少氧化态CBFs:However, after exposure to cold conditions, the demyristoylated Trx-h2 is translocated to the nucleus, where it reduces the oxidized (inactive) CBF oligomers and monomers. The reduced (active) monomers activate cold-regulated gene expression. 结果2-Trx-h2过表达和功能缺失表型:Thus, in contrast to the Arabidopsis trx-h2 (AT5G39950) null mutant, Trx-h2 overexpression lines are highly cold tolerant. 结论:Our findings reveal the mechanism by which cold-mediated redox changes induce the structural switching and functional activation of CBFs, therefore conferring plant cold tolerance. 摘 要
低温响应转录因子CBFs蛋白的活性受到十分严格的调控,因为该转录因子不仅受到低温的诱导,并且还会在温暖的气候条件下调控植物的正常生长。硫氧还蛋白Trx-h2是一种胞质氧化还原蛋白,是CBF1的互作因子,通常通过第二个甘氨酸残基的肉豆蔻酰化锚定在胞质内膜上。但是,在低温条件下,去肉豆蔻酰化的Trx-h2会被转运到细胞核中,从而减少氧化性(惰性态)CBF寡聚体和单体。还原性(激活态)CBF单体能够激活受低温调控的基因的表达。因此,与拟南芥trx-h2功能缺失突变体相反,Trx-h2过表达株系具备较高的低温耐受力。本文的研究结果揭示了低温介导的氧化还原变化诱导了CBFs蛋白的结构转变和功能激活,从而赋予了植株低温抗性。
doi: https://doi.org/10.1038/s41477-021-00944-8
Journal: Nature Plants
Published date: June 21, 2021
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