The de novo genome assembly of Tapiscia sinensis and the transcriptomic and developmental bases of androdioecy

第一作者：Peng Zhao

第一单位：西北大学

通讯作者：Wenzhe Liu

 Abstract 

研究背景：Tapiscia sinensis (Tapisciaceae) possesses an unusual androdioecious breeding system that has attracted considerable interest from evolutionary biologists.

存在问题：Key aspects of T. sinensis biology, including its biogeography, genomics, and sex-linked genes, are unknown.

主要研究：Here, we report the first de novo assembly of the genome of T. sinensis.

结果1-基因组组装、注释：The genome size was 410 Mb, with 22,251 predicted genes.

结果2-群体遗传结构：Based on whole-genome resequencing of 55 trees from 10 locations, an analysis of population genetic structure indicated that T. sinensis has fragmented into five lineages, with low intrapopulation genetic diversity and little gene flow among populations.

结果3-混池测序扫描雄全异株：By comparing whole-genome scans of male versus hermaphroditic pools, we identified 303 candidate sex-linked genes, 79 of which (25.9%) were located on scaffold 25.

结果4-关键位点与转录组：A 24-kb region was absent in hermaphroditic individuals, and five genes in that region, TsF-box4, TsF-box10, TsF-box13, TsSUT1, and TsSUT4, showed expression differences between mature male and hermaphroditic flowers. 

结论：The results of this study shed light on the breeding system evolution and conservation genetics of the Tapisciaceae.

 通讯作者 

doi: 10.1038/s41438-020-00414-w

Journal: Horticulture Research

Published date: Dec 01, 2020