A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress

Xiaoyue Han【韩潇玥】,   Xinyu Song【宋新宇】, Fabiao Yu【于法标】*  and   Lingxin Chen【陈令新】*          

Abstract

Hypothermia and hyperthermia are cell stressed states resulting from environmental temperature changes, which can abnormally decrease intracellular glutathione (GSH) concentrations and horribly induce apoptosis. As the most abundant intracellular nonprotein biothiol, GSH can protect cells from apoptosis. Considering the important roles of GSH in the process of anti-apoptotic in cells and in vivo, we strive to develop a powerful chemical tool for the direct detection of GSH concentration changes under temperature stress. Herein, we report a ratiometric fluorescent probe (CyO-Dise) based on selenium-sulfur exchange reaction for the qualitative and quantitative detection of GSH concentration fluctuations in cells and in vivo. The probe has been successfully used to assess the changes of GSH levels in HepG2 and HL-7702 cells by the stimulations of hypothermia and hyperthermia. In terms of the anti-apoptotic effect of GSH during hypothermia and hyperthermia processes, human normal liver HL-7702 cells have stronger abilities to fight against temperature stress than human liver carcinoma HepG2 cells. Hypothermia and hyperthermia can also improve drug resistance of cis-Dichlorodiamineplatinum(II) (DDP)-resistant HepG2/DDP cells. The probe CyO-Dise has been employed to image GSH concentration changes in HepG2 and HepG2/DDP xenografts nude mice, respectively. With the adjuvant therapy effects of hypothermia and hyperthermia, chemotherapy drug DDP exhibits good ability to therapies of HepG2 and HepG2/DDP xenografts. The above applications make our probe a potential new candidate for accurate diagnosis and efficacy evaluation of cancer.

http://pubs.rsc.org/en/content/articlelanding/2017/sc/c7sc02888a#!divAbstract

A ratiometric fluorescent probe for imaging and quantifying anti-apoptotic effects of GSH under temperature stress

Xiaoyue Han, Xinyu Song, Fabiao Yu and Lingxin Chen    

Chem. Sci., 2017, Accepted Manuscript DOI: 10.1039/C7SC02888A, Edge Article