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第一作者:Katie Abley
第一单位:剑桥大学
通讯作者:James CW Locke
Abstract
背景回顾:Genetically identical plants growing in the same conditions can display heterogeneous phenotypes. 主要研究:Here we use Arabidopsis seed germination time as a model system to examine phenotypic variability and its underlying mechanisms. 结果1-表型变异及位点鉴定:We show extensive variation in seed germination time variability between Arabidopsis accessions and use a multiparent recombinant inbred population to identify two genetic loci involved in this trait. 结果2-两个位点的共同特征:Both loci include genes implicated in modulating abscisic acid (ABA) sensitivity. 结果3-ABA-GA拮抗调控种子萌发时间:Mutually antagonistic regulation between ABA, which represses germination, and gibberellic acid (GA), which promotes germination, underlies the decision to germinate and can act as a bistable switch. 结果4-模型构建:A simple stochastic model of the ABA-GA network shows that modulating ABA sensitivity can generate the range of germination time distributions we observe experimentally. 结果5-模型验证:We validate the model by testing its predictions on the effects of exogenous hormone addition. 结论:Our work provides a foundation for understanding the mechanism and functional role of phenotypic variability in germination time. 摘 要
生长在相同环境条件下、遗传上一致的植株,也能够表现出不一样的表型。本文中,作者利用拟南芥种子萌发时间作为模式系统,研究了表型变异及其潜在的调控机制。作者发现不同拟南芥种质的种子萌发时间存在广泛的变异,利用多亲本重组自交群体鉴定了两个与种子萌发时间相关的遗传位点。这两个位点上都包含参与ABA敏感性的调节基因。ABA抑制种子萌发,而GA促进种子萌发,二者之间的相互拮抗作用作为一个双稳态的开关,共同决定了种子的萌发。作者构建了一个ABA-GA网络的简单随机模型,结果显示调节ABA敏感性可以产生实验上所观察到的发芽时间分布范围。作者进一步通过添加外源激素,然后通过比较模型预测和实际观测之间的差异,验证了模型的有效性。本文的工作为了解植物种子萌发时间这一表型变异的机制和功能提供了基础。
通讯作者
** James CW Locke **
个人简介: 2000年,华威大学,学士; 华威大学,博士。 研究方向:不同的基因表达动态是如何产生的,及其对于生物个体的影响。
doi: https://doi.org/10.7554/eLife.59485
Journal: eLife
Published date: June 01, 2021
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