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第一作者:Jeremy Sloan
Abstract
背景回顾:Stem cells are one of the foundational evolutionary novelties that allowed the independent emergence of multicellularity in the plant and animal lineages. In plants, the homeodomain (HD) transcription factor WUSCHEL (WUS) is essential for the maintenance of stem cells in the shoot apical meristem. WUS has been reported to bind to diverse DNA motifs and to act as transcriptional activator and repressor.
提出问题:However, the mechanisms underlying this remarkable behavior have remained unclear. 主要发现:Here, we quantitatively delineate WUS binding to three divergent DNA motifs and resolve the relevant structural underpinnings. 具体结果:We show that WUS exhibits a strong binding preference for TGAA repeat sequences, while retaining the ability to weakly bind to TAAT elements. This behavior is attributable to the formation of dimers through interactions of specific residues in the HD that stabilize WUS DNA interaction. Fig. 7 DNA-binding behavior of WUS-HD. 结论:Our results provide a mechanistic basis for dissecting WUS dependent regulatory networks in plant stem cell control. 摘 要 干细胞是众多基础的演化新征之一,该特征使得动植物谱系能够相互独立地形成多细胞性。在植物中,同源结构域转录因子WUS对于茎尖和根尖干细胞的维持都是必要的。据报道,WUS能够结合不同的DNA基序,从而发挥其转录激活或抑制的功能。然而,WUS结合DNA基序这一行为背后潜在的机理还不清楚。本文中,作者发现WUS可以结合到三种不同的DNA基序上,并且揭示了相关的结构基础。作者的研究显示WUS存在一个对于TGAA重复序列非常强的结合偏性,同时WUS也存在对于TAAT元件微弱的结合能力。WUS的这种行为可归因于二聚体的形成,该二聚体通过同源结构域上特定的残基互作形成,从而能够稳定WUS与DNA之间的互作。本文的研究为植物干细胞调控中依赖于WUS的调控网络提供了基础。
通讯作者 **Jan U. Lohmann** 研究方向:以拟南芥为模式植物研究植物干细胞调控机制。
doi: 10.1038/s41467-020-16024-y
Journal: Nature Communications
Published date: May 06, 2020
p.s. 往期通讯精彩链接:
Nature Communications:拟南芥WUSCHEL作为生长素响应“变阻器“维持顶端干细胞
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