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Plant Physiology:拟南芥LAZY1基因调控茎秆重力响应方向的分子机制

已有 541 次阅读 2019-12-10 16:01 |个人分类:每日摘要|系统分类:论文交流

Switching the direction of stem gravitropism by altering two amino acids in AtLAZY1

First author: Takeshi Yoshihara; Affiliations: University of Wisconsin (威斯康星大学): Madison, US

Corresponding author: Edgar P. Spalding

From germination to flowering, gravity influences plant growth and development. A rice (Oryza sativa) mutant with a distinctly prostrate growth habit led to the discovery of a gene category that participates in the shaping of plant form by gravity. Each so-called LAZY gene includes five short regions of conserved sequence. The importance of each of these regions in the LAZY1 gene of Arabidopsis thaliana (AtLAZY1) was tested by mutating each region and measuring how well transgenic expression of the resulting protein variant rescued the large inflorescence branch angle of an atlazy1 mutant. The effect of each alteration on subcellular localization was also determined. Region I was required for AtLAZY1 to reside at the plasma membrane, which is necessary for its function. Mutating region V severely disrupted function without affecting subcellular localization. Regions III and IV could be mutated without large impacts on function or localization. Altering region II with two conservative amino acid substitutions (L92A/I94A) had the profound effect of switching shoot gravity responses from negative (upward bending) to positive (downward bending), resulting in a 'weeping' inflorescence phenotype. Mechanical weakness of the stem was ruled out as an explanation for the downward bending. Instead, experiments demonstrated that the L92A/I94A change to AtLAZY1 reversed the auxin gradient normally established across stems by the gravity-sensing mechanism. This discovery opens up new avenues for studying how auxin gradients form across organs and new approaches for engineering plant architecture for agronomic and other practical purposes.



讯:Edgar P. Spalding (https://botany.wisc.edu/staff/spalding-edgar-p/)



doi: https://doi.org/10.1104/pp.19.01144

Journal: Plant Physiology

First Published: December 09, 2019



上一篇:Nature Communications:玉米分蘖决定基因


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