博文
施一公教授的国际学术影响力分析
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发文量 Publications: 288
被引总量 Citations: 7947
科研合作者 Collaborated with 853 co-authors from 1988 to 2012
论文引用者 Cited by 25173 authors
发表的学术论文（被引量大于50次）
- TGF-β signaling controls a plethora of cellular responses and figures prominently in animal development. Recent cellular, biochemical, and structural studies have revealed significant insight into the mechanisms of the activation of TGF-β receptors through ligand binding, the activation of Smad proteins through phosphorylation, the transcriptional regulation of target gene expression, and the control of Smad protein activity and ...
- Caspases are central components of the machinery responsible for apoptosis. Recent structural and biochemical studies on procaspases, IAPs, Smac/DIABLO, and apoptosome have revealed a conserved mechanism of caspase activation and inhibition. This article reviews these latest advances and presents our current understanding of caspase regulation during apoptosis.
- Caspases, which are the executioners of apoptosis, comprise two distinct classes, the initiators and the effectors. Although general structural features are shared between the initiator and the effector caspases, their activation, inhibition and release of inhibition are differentially regulated. Biochemical and structural studies have led to important advances in understanding the underlying molecular mechanisms of caspase regulation. This article reviews ...Journal: Nature Reviews Molecular Cell Biology - NAT REV MOL CELL BIOL, vol. 5, no. 11, pp. 897-907, 2004
- Zinc ions are key structural components of a large number of proteins. The binding of zinc stabilizes the folded conformations of domains so that they may facilitate interactions between the proteins and other macromolecules such as DNA. The modular nature of some of these zinc-containing proteins has allowed the rational design of site-specific DNA binding proteins. The ability ...
- Eric N Shiozaki, Jijie Chai, Daniel J Rigotti, Stefan J Riedl, Pingwei Li, Srinivasa M Srinivasula, Emad S Alnemri, Robert Fairman,The inhibitor of apoptosis (IAP) proteins potently inhibit the catalytic activity of caspases. While profound insight into the inhibition of the effector caspases has been gained in recent years, the mechanism of how the initiator caspase-9 is regulated by IAPs remains enigmatic. This paper reports the crystal structure of caspase-9 in an inhibitory complex with the third baculoviral ...
- The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 Å resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making extensive contacts to the residues that are essential...
- Apoptosis-inducing factor (AIF), a mitochondrial oxidoreductase, is released into the cytoplasm to induce cell death in response to apoptotic signals. However, the mechanisms underlying this process have not been resolved. We report that inactivation of the Caenorhabditis elegans AIF homolog wah-1 by RNA interference delayed the normal progression of apoptosis and caused a defect in apoptotic DNA degradation. ...
- The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. HAUSP, a representative UBP, specifically deubiquitinates and hence stabilizes the tumor suppressor protein p53. Here, we report the crystal structures of the 40 kDa catalytic core domain of HAUSP in isolation and in complex with ubiquitin aldehyde. These studies reveal that the ...
- Jie-Oh Lee, Haijuan Yang, Maria-Magdalena Georgescu, Antonio Di Cristofano, Tomohiko Maehama, , Jack E Dixon, Pier Pandolfi, Nikola P PavletichThe PTEN tumor suppressor is mutated in diverse human cancers and in hereditary cancer predisposition syndromes. PTEN is a phosphatase that can act on both polypeptide and phosphoinositide substrates in vitro. The PTEN structure reveals a phosphatase domain that is similar to protein phosphatases but has an enlarged active site important for the accommodation of the phosphoinositide substrate. The structure ...
- Apoptosis is executed by caspases, which undergo proteolytic activation in response to cell death stimuli. The apoptotic protease-activating factor 1 (Apaf-1) controls caspase activation downstream of mitochondria. During apoptosis, Apaf-1 binds to cytochrome c and in the presence of ATP/dATP forms an apoptosome, leading to the recruitment and activation of the initiator caspase, caspase-9 (ref. ...
- Apoptosome refers to the adaptor protein complex that mediates the activation of an initiator caspase at the onset of apoptosis. In mammalian cells, caspase-9, caspase-8, and caspase-2 rely on the apoptotic protease-activating factor 1 (Apaf-1)-apoptosome, death-inducing signaling complex (DISC), and PIDDosome, respectively, for activation. In Drosophila, activation of the caspase-9 homolog Dronc ...Journal: Cell Death and Differentiation - CELL DEATH DIFFERENTIATION, vol. 14, no. 1, pp. 56-65, 2007
- Signal transduction by the TGF-b family involves sets of receptor serine/threonine kinases, Smad proteins that act as receptor substrates, and Smad-associated transcription factors that target specific genes. We have identified discrete structural elements that dictate the selective interactions between receptors and Smads and between Smads and transcription factors in the TGF-b and BMP pathways. A cluster ...Published in 1998.
- Geng Wu, Ye-Guang Chen, Barish Ozdamar, Cassie A. Gyuricza, P. Andrew Chong, Jeffrey L. Wrana, Joan Massagué,The Smad proteins mediate transforming growth factor-beta (TGFbeta) signaling from the transmembrane serine-threonine receptor kinases to the nucleus. The Smad anchor for receptor activation (SARA) recruits Smad2 to the TGFbeta receptors for phosphorylation. The crystal structure of a Smad2 MH2 domain in complex with the Smad-binding domain (SBD) of SARA has been determined at 2.2 angstrom ...
- Journal: Journal of The National Cancer Institute - J NAT CANCER INST, vol. 90, no. 12, pp. 916-920, 1998
- Yanhui Xu, Yongna Xing, Yu Chen, Yang Chao, Zheng Lin, Eugene Fan, Jong W. Yu, Stefan Strack, Philip D. Jeffrey,Protein Phosphatase 2A (PP2A) plays an essential role in many aspects of cellular physiology. The PP2A holoenzyme consists of a heterodimeric core enzyme, which comprises a scaffolding subunit and a catalytic subunit, and a variable regulatory subunit. Here we report the crystal structure of the heterotrimeric PP2A holoenzyme involving the regulatory subunit B'/B56/PR61. Surprisingly, the B'/PR61 subunit ...
- , C. Mello
- The 1.9 Å crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis(Citations: 66)
The 1.9 Å X-ray structure of a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis is reported. This enzyme, MurG, contains two a0b open sheet domains separated by a deep cleft. Structural analysis suggests that the C-terminal domain contains the UDP-GlcNAc binding site while the N-terminal domain contains the acceptor binding site and likely membrane association... - Nieng Yan, Jijie Chai, Eui Seung Lee, Lichuan Gu, Qun Liu, Jiaqing He, Jia-Wei Wu, David Kokel, Huilin Li, Quan Hao, Ding Xue,Interplay among four genes-egl-1, ced-9, ced-4 and ced-3-controls the onset of programmed cell death in the nematode Caenorhabditis elegans. Activation of the cell-killing protease CED-3 requires CED-4. However, CED-4 is constitutively inhibited by CED-9 until its release by EGL-1. Here we report the crystal structure of the CED-...
- Multiple Apoptotic Caspase Cascades Are Required in Nonapoptotic Roles for Drosophila Spermatid Individualization(Citations: 64)Spermatozoa are generated and mature within a germline syncytium. Differentiation of haploid syncytial spermatids into single motile sperm requires the encapsulation of each spermatid by an independent plasma membrane and the elimination of most sperm cytoplasm, a process known as individualization. Apoptosis is mediated by caspase family proteases. Many apoptotic cell deaths in Drosophila utilize the REAPER/HID/GRIM family ...
- Xia Lin, Xueyan Duan, Yao-Yun Liang, Ying Su, Katharine H. Wrighton, Jianyin Long, Min Hu, Candi M. Davis, Jinrong Wang, F. Charles Brunicardi, , Ye-Guang Chenhttp://academic.research.microsoft.com/io.ashx?type=5&id=5984841&selfId1=1305616&selfId2=-1&maxNumber=12&query=
- The inhibitor of apoptosis protein DIAP1 suppresses apoptosis in Drosophila, with the second BIR domain (BIR2) playing an important role. Three proteins, Hid, Grim, and Reaper, promote apoptosis, in part by binding to DIAP1 through their conserved N-terminal sequences. The crystal structures of DIAP1-BIR2 by itself and in complex with the N-terminal peptides from Hid and Grim ...
- The inhibitor of apoptosis protein DIAP1 inhibits Dronc-dependent cell death by ubiquitinating Dronc. The pro-death proteins Reaper, Hid and Grim (RHG) promote apoptosis by antagonizing DIAP1 function. Here we report the structural basis of Dronc recognition by DIAP1 as well as a novel mechanism by which the RHG proteins remove DIAP1-mediated downregulation of Dronc. Biochemical and structural ...
- Transforming growth factor beta (TGF-beta ) regulates a broad range of biological processes, including cell growth, development, differentiation, and immunity. TGF-beta signals through its cell surface receptor serine kinases that phosphorylate Smad2 or Smad3 proteins. Because Smad3 and its partner Smad4 bind to only 4-bp Smad binding elements (SBEs) in DNA, a central question is how specificity of ...Journal: Proceedings of The National Academy of Sciences - PNAS, vol. 961, no. 23, pp. 13130-13135, 1999
- Carbonic anhydrase, a zinc enzyme found in organisms from all kingdoms, catalyses the reversible hydration of carbon dioxide and is used for inorganic carbon acquisition by phytoplankton. In the oceans, where zinc is nearly depleted, diatoms use cadmium as a catalytic metal atom in cadmium carbonic anhydrase (CDCA). Here we report the crystal structures of CDCA in four distinct forms: ...
- Apoptosis (programmed cell death), an essential process in the development and homeostasis of metazoans, is carried out by caspases. The mitochondrial protein Smac/DIABLO performs a critical function in apoptosis by eliminating the inhibitory effect of IAPs (inhibitor of apoptosis proteins) on caspases. Here we show that Smac/DIABLO promotes not only the proteolytic activation of procaspase-3 but also ...
- Jia-Wei Wu, Min Hu, Jijie Chai, Joan Seoane, Morgan Huse, Carey Li, Daniel J. Rigotti, Saw Kyin, Tom W. Muir, Robert Fairman, Joan Massagué,Ligand-induced phosphorylation of the receptor-regulated Smads (R-Smads) is essential in the receptor Ser/Thr kinase-mediated TGF-β signaling. The crystal structure of a phosphorylated Smad2, at 1.8 Å resolution, reveals the formation of a homotrimer mediated by the C-terminal phosphoserine (pSer) residues. The pSer binding surface on the MH2 domain, frequently targeted for inactivation...
- Transforming Growth Factor -Mediated Transcriptional Repression of c-myc Is Dependent on Direct Binding of Smad3 to a Novel Repressive Smad Binding Element(Citations: 50)Smad proteins are the most well-characterized intracellular effectors of the transforming growth factor (TGF-) signal. The ability of the Smads to act as transcriptional activators via TGF--induced recruitment to Smad binding elements (SBE) within the promoters of TGF- target genes has been firmly established. However, the elucidation of the molecular mechanisms involved in TGF--mediated transcriptional repression are ...
施一公的事迹十分感人
http://blog.sciencenet.cn/blog-280034-693530.html
预祝施一公教授当选中国科学院院士
http://blog.sciencenet.cn/blog-280034-691951.html
| 央视《新闻联播》介绍施一公全职回国工作 |
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1985年，施一公被保送进入清华大学生物系。1989年，他又以年级第一名的成绩提前一年毕业，去美国留学。
就这样，一边打工赚钱，一边要在实验室中寻找自己的研究目标。在读博士后期间，施一公确定了“癌症发生和细胞凋亡”这个研究方向。
因为在这一领域的贡献，2003年，年仅36岁的施一公获得全球生物学界享有盛名的“鄂文西格青年科学家奖”，并成为普林斯顿大学分子生物学系历史上最年轻的终身教授。
十多年前出国时做的求学梦，已经完全变成了现实。但这时的施一公总还是觉得心里缺点什么。一个更大的梦想、更大的挑战，就要走到他的面前。
仅仅一夜间，就决定放弃令人羡慕的优厚条件全职回国，没提任何条件就要从头开始建立实验室，施一公的选择立刻遭到很多人的劝阻。
施一公心里清楚，这时的梦，已不是学术地位或是优厚条件，而是自己要为那片生他养他的土地，做点事情。他用两年的过渡期关掉了美国的实验室，回到清华开始了第二次创业。从买仪器设备，到招聘研究人员，一切从头再来。这个创业梦，不止是施一公一个人的。清华大学也想方设法给他“开绿灯”，帮着一块使劲儿。
短短5年，施一公又先后把70多名世界范围的优秀人才引回清华大学全职工作。他本人也在2010年把自己的国籍从美国改回了中国。
回国后这5年，施一公又在《自然》等国际顶级期刊上发表了12篇论文。高水平成果产出的频率，比他在国外时还要高。2010年，一支世界一流学者组成的国际评估组来到清华，评估的结果是：清华大学生命学科发展态势很好，其中结构生物学学科已达到世界一流水平。（原标题：【出彩人生：中国梦·我的梦】施一公：“海归”生物学家圆梦祖国）
http://news.sciencenet.cn/htmlnews/2013/5/278298.shtm
https://blog.sciencenet.cn/blog-280034-693837.html
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