A novel ESIPT-ICT probe for fluorescent assessment and imaging of serum albumin via esterase-like activity发布时间：2025-11-09 发布者： 浏览次数：1

Dyes and Pigments

Volume 246, Part 1, March 2026, 113385

https://doi.org/10.1016/j.dyepig.2025.113385

A novel ESIPT-ICT probe for fluorescent assessment and imaging of serum albumin via esterase-like activity

Author links open overlay panelMei Yang a,Wei Song b,Bing Yang a,Yanni Wang a,Fabiao Yu c d

• aSchool of Pharmaceutical Sciences, Guizhou University, Guiyang, 550025, China

• bThe First Affiliated Hospital of Dalian Medical University, Dalian, 116023, China

• cNHC Key Laboratory of Tropical Disease Control, Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Hainan Functional Materials and Molecular Imaging, School of Life Sciences and Medical Technology, Hainan Medical University, Haikou, Hainan, 571199, China

• dKey Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Haikou Trauma, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital, Hainan Medical University, Haikou, 571199, China

Received 9 October 2025, Revised 28 October 2025, Accepted 2 November 2025, Available online 4 November 2025, Version of Record 7 November 2025.

Highlights

• •Evaluation of the functional level of human serum albumin (HSA) through its esterase-like activity (HSAEA).

  •THOA enables precise, dynamic, and high-throughput measurement of HSAEA.

• •THOA identifies subclinical states with normal HSA binding but impaired HSAEA function in serum tests.

• •THOA is applicable to fluorescence imaging in live cells and zebrafish.

Abstract

Human serum albumin (HSA) exhibits diverse enzymatic properties, with its esterase-like activity (HSAEA) playing a pivotal role in lipid metabolism and drug development processes. Herein, we developed a novel fluorescent probe, THOA, for the specific evaluation of HSAEA and albumin. Upon cleavage by HSAEA, THOA undergoes hydrolysis to yield THOH, which demonstrates heightened fluorescence intensity through a synergistic mechanism involving ESIPT and ICT. The THOA probe demonstrated outstanding selectivity, precise triple-linear response, and reliable performance in the quantification of HSAEA. When applied to serum samples from both healthy volunteers and nephrology patients, THOA showed a unique capability to identify subclinical cases characterized by normal binding capacity but aberrant HSAEA activity. Additionally, THOA was successfully employed for in-situ imaging of reabsorbed HSA in cells and zebrafish. This study provides crucial insights into assessing the functional status of HSA.

Graphical abstract

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