OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8

第一作者：Qi Wu

讯通单位：重庆大学

通讯作者：黄俊丽

理论视角：Ideal plant architecture and drought tolerance are important determinants of yield potential in rice (Oryza sativa). Here, we found that OsNAC016, a rice stress-responsive NAC transcription factor, functions as a regulator in the crosslink between BR-mediated plant architecture and ABA-regulated drought responses. 

理想株型和耐旱能力是水稻产量潜力的重要因素。该研究发现水稻胁迫响应NAC转录因子OsNAC016在BR介导的植株和ABA介导干旱响应之间起调节作用。

The loss-of-function mutant osnac016 exhibited erect leaves and shortened internodes, but OsNAC016-overexpressing plants had opposite phenotypes. Further investigation revealed that OsNAC016 regulated the expression of the brassinosteroid biosynthesis gene D2 by binding to its promoter. Moreover, OsNAC016 interacted with and was phosphorylated by GSK3/SHAGGY-LIKE KINASE2 (GSK2), a negative regulator in the BR pathway. 

功能缺失的突变体osnac016表现出直立叶和短节间，但是过表达OsNAC016则出现相反表型。进一步探究揭示了OsNAC016通过与其启动子结合来调控油菜素内酯生物合成基因D2的表达。此外，OsNAC016与BR途径中的负调控因子GSK2互作和并被其磷酸化。

Meanwhile, the mutant osnac016 had improved drought stress tolerance, supported by a decreased water loss rate and enhanced stomatal closure in response to exogenous ABA, but OsNAC016-overexpressing plants showed attenuated drought tolerance and reduced ABA sensitivity. Further, OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE8 (SAPK8) phosphorylated OsNAC016 and reduced its stability. The ubiquitin/26S proteasome system is an important degradation pathway of OsNAC016 via the interaction with PLANT U-BOX PROTEIN43 (OsPUB43) that mediates the ubiquitination of OsNAC016. 

同时，突变体osnac016减少水分损失率，气孔关闭，增强响应外源ABA而提升耐寒性，而过表达OsNAC016减少ABA敏感性。进一步SAPK8磷酸化OsNAC016降低其敏感性。26S蛋白酶体系统通过与OsPUB43互作是OsNAC016重要的降解途径，介导OsNAC016泛素化。

Notably, RNA-sequencing analysis revealed global roles of OsNAC016 in promoting BR-mediated gene expression and repressing ABA-dependent drought-responsive gene expression, which was confirmed by ChIP-qPCR analysis. Our findings establish that OsNAC016 is positively involved in BR-regulated rice architecture, negatively modulates ABA-mediated drought tolerance, and is regulated by GSK2, SAPK8, and OsPUB43 through post-translational modification. 

值得注意的是，转录组分析揭示 OsNAC016促进BR-介导基因表达并且抑制ABA依赖的干旱反应的基因表达，这一点得到ChIP-qPCR分析验证。我们的研究构建OsNAC016是正向调控BR介导水稻株型，负调控ABA介导的耐旱性，并通过转录修饰受GSK2, SAPK8和 OsPUB43的调控。

结论：Our data provide insights into how plants balance growth and survival by coordinately regulating the growth-promoting signaling pathway and response under abiotic stresses.

我们的结果为植物如何协调的调节促进生长信号通路和非生物胁迫响应来平衡生长和生存提供思路。

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**黄俊丽**

研究方向：1. 分子生物学与基因工程；2. 植物生长发育调控；3. 植物逆境响应机制

2003年毕业于中国农业大学获植物病理专业博士学位，2008年-2009年在美国普渡大学进行访问研究

Journal: Plant Physiology, 

Published data: 25 March 2022