Life = f(Environment, t) is far from a simple mathematical expression; it is a powerful scientific framework that unifies ancient philosophical wisdom with cutting-edge life science technology. This report systematically demonstrates:

1.     Theoretical Elevation: The formula refines the holistic concept of "Harmony between Heaven and Humanity" into a rigorous framework containing four key elements—the living subject, environmental factors, temporal dynamics, and interaction mechanisms—amenable to scientific investigation.

1.     Language Establishment: Ionic & Molecular Omics (imOmics), using ion/molecule flux as its core metric, provides the scientific language for quantifying and interpreting the dynamic interactions (the function "f") between life and environment.

1.     Tool Implementation: The technology system centered on NMT enables the in vivo, real-time, in situ measurement of this dynamic process, putting theory into practice.

1.     Practical Validation: The Global imOme Project (GiP), through the establishment of a global standardized network and its applications in smart agriculture, precision medicine, environmental monitoring, and other fields, systematically and on a large scale validates and applies the effectiveness and immense potential of the Life = f(Environment, t) formula.

1.     New Biology Dogma: The traditional Central Dogma of molecular biology describes the linear flow of genetic information from DNA to RNA to protein, laying the foundation for the life sciences. However, this framework primarily focuses on the encoding and expression of information within the cell, failing to fully reveal how living organisms, as dynamic open systems, integrate internal instructions with the external environment through continuous ion and molecule exchange to ultimately achieve complex phenotypes. The newly proposed "Dogma": DNA → RNA → Protein → imOme → Phenotype, serves as an important supplement to this limitation. It extends the molecular pathway of the Central Dogma to the macroscopic dynamic level of the ion-molecule group (imOme) and finally connects it to the phenotype, constructing a more complete system for describing life activities that emphasizes real-time interaction between the organism and its environment.

Figure 3. New Biology Dogma. imOme: refers to the dynamic totality of all ion and molecule exchange activities between an organism (and its components) and the external environment under specific time and conditions. It functions like a real-time log of life's interactions, documenting how an organism regulates the fluxes of ions and molecules in response to environmental changes, maintains internal homeostasis, and carries out various life processes. It expands the research perspective from the internal transmission of genetic information within the cell to the real-time material and energy exchange processes of the entire organism within its environment. Understanding the imOme as a bridge connecting microscopic molecular events to macroscopic phenotypes holds significant value for elucidating the mechanisms of complex diseases, developing novel environmental remediation strategies, and improving agricultural product quality, among other fields. This marks a transition for the life sciences from deciphering the "book of life" based on sequences to monitoring and interpreting the real-time "flow of life".

The core of this new paradigm lies in positioning the imOme as a critical link connecting upstream and downstream. Proteins, as the end products of gene expression, constitute the executors of life activities, but they do not function in a vacuum. The imOme precisely captures the dynamic sum of all ion and molecule exchanges between an organism and its external environment at a specific time and space, mediated and regulated by proteins (such as ion channels, transporters, enzymes, etc.). This process is like a real-time "log of life interactions." It not only records the static blueprint based on genetic information but also dynamically reflects how the organism responds to environmental changes like light, temperature, nutrients, and even stress by regulating the flux, and distribution of ions (e.g., K⁺, Ca²⁺) and molecules (e.g., glucose, metabolites). This dynamic exchange maintains internal homeostasis and drives various life activities such as growth, development, and adaptation.