2025年12月10日，国际知名期刊《Analytical Chemistry》在线发表了云南师范大学化学化工学院杨建梅与西南大学向云教授合作取得的最新研究成果《Highly Catalytic FeS2@CoS2 Core/Shell Nanocubes for Ultrasensitive Electrochemical Assay of Microphthalmia-Associated Transcription Factor via Nucleic Acid Signal Amplification Cascades》（中国科学院一区TOP，影响因子IF=6）论文链接：https://pubs.acs.org/doi/10.1021/acs.analchem.5c04603，云南师范大学化学化工学院杨建梅与西南大学向云教授共同通讯作者。

Dysregulation expression of microphthalmia-associated transcription factor (MITF), a principal melanocyte controller, has been closely linked to melanin-associated disorders, and developing highly sensitive MITF detection methods is therefore imperative for early diagnosis and prognosis monitoring of melanoma-associated symptoms. This work describes an ultrasensitive electrochemical MITF biosensor on the basis of highly catalytic FeS2@CoS2 core/shell nanocubes and target recycling/bipedal DNA walker signal amplification cascades. The FeS2@CoS2 nanocubes are prepared with the one-step simple hydrothermal means, followed by their modification on the electrode, electrodeposition of AuNPs, and immobilization of DNA capture probes to prepare the sensing interface. Target MITF molecules bind and protect the dsDNA recognition duplexes from digestion by exonuclease III (Exo III) to trigger subsequent target recycling/bipedal DNA walker reaction cascades. This leads to confining a lot of ferrocene (Fc)-modified DNAs to the electrode sensing surface. FeS2@CoS2-mediated catalytic oxidation of Fc thus generates significantly amplified current signals, achieving MITF detection across 0.5 ∼ 1000 pM with the remarkable 0.12 pM detection limit. Moreover, such a biosensor shows excellent selectivity and can realize trace MITF detection in cancer cell nuclear extracts, demonstrating its potential as a universal platform for trace

https://pubs.acs.org/doi/10.1021/acs.analchem.5c04603

杨建梅                        

分析化学专业，讲师，硕士生导师

联系方式：yangatynnu@163.com

 学术任职及兼职

化学化工学院教师

 主要研究方向

纳米材料及生物传感器

 主要招生专业

分析化学

 教育与工作经历:

2011年9月～2015年6月，云南师范大学 化学化工学院 化学，学士

2015年9月～2020年6月，西南大学 化学化工学院 分析化学 博士

获奖成果和荣誉称号：

杨建梅. 云南省“兴滇英才支持计划”——青年人才专项，2022年

 主持科研项目

1. 云南省基础研究计划项目（面上项目），高灵敏、高稳定性microRNA电化学生物传感器的构建研究，执行时间：2023/06-2026/05

2. 云南省基础研究计划项目（青年项目），光刺激可再生防污癌症标志物电化学生物传感器的研制及应用，执行时间：2022/06-2025/05

3. 云南省教育厅科学基金项目，血糖仪用于癌症标志物的即时检测，执行时间：2021/03-2022/02。

代表性著作、论文、专利等

1. Jian-Mei Yang, Xiao-Long Li, Bing-Ying Jiang*, Ruo Yuan, Yun Xiang*. In situ-generated multivalent aptamer network for efficient capture and sensitive electrochemical detection of circulating tumor cells in whole blood. Analytical Chemistry, 2020, 92(11), 7893-7899.

2. Jian-Mei Yang, Bao-Ting Dou, Ruo Yuan, Yun Xiang*. Proximity binding and metal ion-dependent DNAzyme cyclic amplification-integrated aptasensor for label-free and sensitive electrochemical detection of thrombin. Analytical Chemistry, 2016, 88(16), 8218-8223.

3. Jian-Mei Yang, Bao-Ting Dou, Ruo Yuan, Yun Xiang*. Aptamer/protein proximity binding-triggered molecular machine for amplified electrochemical sensing of thrombin. Analytical Chemistry, 2017, 89(9), 5138-5143.

4. Jian-Mei Yang, Yun-Lan Wu, Chun-Fang Gan*, Ruo Yuan, Yun Xiang*. Target-programmed and autonomous proximity binding aptasensor for amplified electronic detection of thrombin. Biosensors and Bioelectronics, 2018, 117, 743-747.

5. Jian-Mei Yang, Xiao-Tong Huang, Chun-Fang Gan*, Ruo Yuan, Yun Xiang*. Highly specific and sensitive point-of-care detection of rare circulating tumor cells in whole blood via a dual recognition strategy. Biosensors and Bioelectronics, 2019, 143, 111604.

6. Jian-Mei Yang*, Qian Bi, Xin-Mei Song, Ruo Yuan, Yun Xiang*. Aptamer/organometallic receptor-based and label-free sensitive electrochemical detection of amino acid via multi-pedal DNA walker/DNAzyme amplifications. Sensors & Actuators: B. Chemical, 2023,375, 132876.

7. Jian-Mei Yang*, Xiao-Yan Gan, Xin-Mei Song, Ruo Yuan, Yun Xiang*. Apamer-based sensitive and label-free electrochemical detection of neutrophil gelatinase-associated lipocalin via recycling amplification cascades. Analytica Chimica Acta, 2022, 1233, 340515.

8. Lingl-Li Wang, Teng-Teng Shan, Lu Pu, Jin Zhang, Rong Hu, Yun-Hui Yang, Jian-Mei Yang*, Yan Zhao*. Glucometer-based electrochemical biosensor for determination of microRNA (let-7a) using magnetic-assisted extraction and supersandwich signal amplifcation. Microchimica Acta, 2022, 189, 444.

9. Yu-Long Li, Fa-Ting Xie, Cao Yao, Gui-Qun Zhang, Yan Guan, Yun-Hui Yang, Jian-Mei Yang*, Rong Hu*. A DNA tetrahedral nanomaterial-based dual-signal ratiometric electrochemical aptasensor for the detection of ochratoxin A in corn kernel samples. Analyst, 2022, 147, 4578-4586.

10. Ling-Li Wang, Hui-Wen Yang, Fang-Dao Zhu, Shao-Ming Chi, Jin Zhang, Jian-Mei Yang*, Yan Zhao*, Host-guest inclusion systems of two bioactive natural products derivantives and three polyamine-modified β-cyclodextrins: Preparation, characterization, biological activity. Journal of Drug Delivery Science and Technology, 2022, 67, 102940.

主讲课程

本科生课程《分析化学》、《分析化学实验》、《仪器分析》、《仪器分析实验》