氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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氢气治疗大鼠心肌梗死

已有 6789 次阅读 2009-6-15 18:24 |个人分类:氢气生理盐水|系统分类:科研笔记|关键词:氢分子医学,心脏疾病| 氢分子医学, 心脏疾病

这个文章在6月12日被接受。是我们的第6篇,是世界上的17篇文章。目前全世界这个领域已经达到18篇论著。

文章采用最常规的方法:制备心肌梗死动物模型(这个比较麻烦一些),给氢盐水,观察功能、梗死体积和细胞损伤等常见指标,结合氧化损伤指标测定。

而且这个方面日本已经有论文发表,我们新在给氢方式上,审稿专家给的意见比较好。可惜杂志的发表周期很长,否则早就发表了。我们是去年的实验。

到目前我们已经发表了4篇采用注射方法的论文,也是目前唯一发表的4篇。

 氢气饱和盐水可保护(大鼠)心肌的缺血/再灌注损伤

摘要

此前的研究表明,H2在心肌的缺血/再灌注损伤中具有保护作用。本研究旨在验证如下假设――价廉物美、操作简单的氢气饱和盐水可保护大鼠心肌受缺血(30分钟)/再灌注(24小时)所致的损伤。成年雄性SD大鼠夹闭左冠状动脉30分钟/再灌注24小时。在再灌注前,腹腔注射氢气饱和盐水后能明显降低大鼠血浆及心肌中的丙二醛(MDA)浓度、减少心肌细胞的凋亡和降低AAR区的8羟基鸟嘌呤核苷水平、抑制caspase-3的活化和减少梗死面积。再灌注24小时后,包括LVSP、LVDP的+(dP/dt)及-(dP/dt) 最大值等反映心功能的参数也明显改善。结论:(给药)氢气饱和盐水是一种新型、简单、安全、有效的缓解心肌缺血/再灌注损伤的方法。

关键词:再灌注,氧化应激,富含H的盐,凋亡

 Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats
Qiang Sun 1, Zhimin Kang 1, Jianmei Cai 1, Wenwu Liu 1, Yun Liu 1, John H Zhang 2, Petar J Denoble 3, Hengyi Tao 1, and Xuejun Sun 1*
1 Second Military Medical University
2 Division of Neurosurgery, Loma Linda University, Loma Linda, California, USA
3 c Divers Alert Network-an associate of Duke University Health System

* To whom correspondence should be addressed. E-mail: sunxjk@hotmail.com.

Abstract

Protective effect of hydrogen (H2) gas on cardiac ischemia-reperfusion (I/R) injury has been demonstrated previously. This study was designed to test the hypothesis that hydrogen-rich saline (saline saturated with molecular hydrogen) which is easy to use induces cardioprotection against ischemia (30 min) and reperfusion (24 h) injury in rats. Adult male Sprague-Dawley rats underwent 30 minute occlusion of the left anterior descending (LAD) coronary artery and 24 hour reperfusion. Intraperitoneal injection of hydrogen-rich saline before reperfusion significantly decreased plasma and myocardium malondialdehyde (MDA) concentration, decreased cardiac cell apoptosis and myocardial 8-hydroxydeoxyguanosine (8-OHdG) in area at risk zonesAAR, suppressed the activity of caspase 3, and reduced infarct size. The heart function parameters including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP)+(dP/dt)max and -(dP/dt)max were also significantly improved 24 h after reperfusion. It is concluded that hydrogen-rich saline is a novel, simple, safe and effective method to attenuate myocardial I/R injury.

Key Words: reperfusion, oxidative stress, hydrogen-rich saline, apoptosis

 

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