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氢气治疗结肠炎
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这个领域正想我2年前估计的那样，开始快速发展了，这个研究是针对人类一个非常普遍的疾病：结肠炎，这个疾病不厉害，但严重困扰许多人的生活。有这个病的人都有体会。而且治疗手段非常缺乏。主要是病因不清楚。但炎症反应是非常确定的。氢治疗该疾病只针对炎症，虽然不能针对病因。但也提供了一个非常值得考虑的方法。这个研究非常值得重视。预示着氢分子医学向更广泛领域的发展。而且更具有实际意义。
Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate
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Mikihito Kajiyaa, Marcelo J.B. Silvaa, Kimihiro Satob, Kazuhisa Ouharaa and Toshihisa Kawaia, 
, 
Received 18 May 2009.
Abstract
By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.
Keywords: Molecular hydrogen; Colitis; Inflammation; Antioxidant; Colon; Dextran sodium sulfate; IL-12; TNF-alpha; IL-1-beta; Macrophages
Article Outline
Fig. 1. Effects of H2 on the clinical features of DSS-induced colitis in BALB/c mice. Temporal change of H2 concentration in stomach (A) and in colon (B) after the oral administration of H2-saturated water to mice (n = 5/each time point) was monitored. Distilled water saturated with H2 (0.78 mM, ORP = −511 mV, pH 7.67) was applied to mice using a Popper® feeding needle (1 ml/mouse). Immediately after the sacrifice of animals in each group, H2 concentrations of liquid substance present in stomach and colon were measured. To examine the effects of H2 on DSS-induced colitis, H2 (0.78 mM) water alone or 5% DSS with or without H2-water were administered to mice (8 w males, n = 6/group) ad libitum for 7 days. The percent of body weight change (C) and colitis score (D) were measured on a daily basis. Data points and bars of H2-water alone (♦), 5% DSS water alone (□), and 5% DSS including H2-water (
) indicate means ± SD. *p < 0.05, **p < 0.01: Value is significantly different from the control group receiving DSS alone in the same measurement day (t-test).
Fig. 2. H2-mediated alleviation of colon contraction induced by DSS. To assess the severity of colitis, regular water, H2 (0.78 mM) water alone, or 5% DSS with or without H2-water were administered to mice for 7 days after which the colon length was measured. (A) Representative macroscopic features of the colons isolated from mice treated with regular water (Control), H2-containing water (H2), 5% DSS water (DSS) and 5% DSS containing H2-water (H2 + DSS) are shown. (B) The measurement of mean ± SD of colon length in each group at Day-7 is depicted. **p < 0.01: Value differs significantly between the groups indicated by brackets (t-test).
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