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博文

携带自然重组核蛋白基因的新型狂犬病毒世系的分离和鉴定

已有 4329 次阅读 2012-12-19 11:18 |个人分类:RV流行病学|系统分类:论文交流| 狂犬病, 基因, 自然重组, 核蛋白, 世系

论文题目:Isolation and Identification of a Novel Rabies Virus Lineage in China with Natural Recombinant Nucleoprotein Gene

发表刊物: PLoS ONE 7(12): e49992. doi:10.1371/journal.pone.0049992

 

论文摘要:

狂犬病病毒(RABV)引起严重的神经系统疾病和死亡。作为导致病毒遗传多样性的一种重要机制,同源重组可能导致毒力增加和宿主趋向性有改变的新病毒株的出现。然而,此前尚不清楚在RABV的进化中重组是否在起作用。在本研究中,我们对在中国流行的4RABV毒株进行分离和测序。系统进化分析确认了由2种不同毒株(毒株JCQ92)构成的一个新的世系(lineage),该世系可能源于杂交。进一步的分析表明,该世系病毒基因组的3’端非翻译区(UTR)N基因的一部分(长度为大约500个碱基)很可能来源中国世系I,而该基因组序列的其他部分则与中国世系II同源。我们的研究结果显示,同源重组可以在环境中自然发生,并参与决定RABV种群的基因结构。

 

Abstract

Rabies virus (RABV) causes severe neurological disease and death. As an important mechanism for generating genetic diversity in viruses, homologous recombination can lead to the emergence of novel virus strains with increased virulence and changed host tropism. However, it is still unclear whether recombination plays a role in the evolution of RABV. In this study, we isolated and sequenced four circulating RABV strains in China. Phylogenetic analyses identified a novel lineage of hybrid origin that comprises two different strains, J and CQ92. Analyses revealed that the virus 3’ untranslated region (UTR) and part of the N gene (approximate 500 nt in length) were likely derived from Chinese lineage I while the other part of the genomic sequence was homologous to Chinese lineage II. Our findings reveal that homologous recombination can occur naturally in the field and shape the genetic structure of RABV populations.

 

论文作者:

Cheng-Qiang HeSheng-Li MengHong-Yan YanNai-Zheng DingHong-Bin HeJia-Xin YanGe-Lin Xu

作者单位:

1.  Key Laboratory of Systems Biology in Universities of Shandong, College of Life Science, Shandong Normal University, Jinan, China.

2.  Wuhan Institute of Biological Products, Wuhan, China.

3.  Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, China.


原文全文下载:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049992 

或:He-journal.pone.0049992.pdf




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