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Genome Research:温度胁迫加速拟南芥的突变速率

已有 634 次阅读 2020-12-30 10:06 |个人分类:每日摘要|系统分类:论文交流

Thermal stress accelerates Arabidopsis thaliana mutation rate

第一作者Eric J. Belfield

第一单位牛津大学

通讯作者Nicholas P. Harberd


 Abstract 


背景回顾Mutations are the source of both genetic diversity and mutational load.


提出问题:However, the effects of increasing environmental temperature on plant mutation rates and relative impact on specific mutational classes (e.g., insertion/deletion [indel] vs. single nucleotide variant [SNV]) are unknown.


课题重要性:This topic is important because of the poorly defined effects of anthropogenic global temperature rise on biological systems.


主要研究:Here, we show the impact of temperature increase on Arabidopsis thaliana mutation, studying whole genome profiles of mutation accumulation (MA) lineages grown for 11 successive generations at 29°C.


结果1-高温与常温下的突变率:Whereas growth of A. thaliana at standard temperature (ST; 23°C) is associated with a mutation rate of 7 × 10−9 base substitutions per site per generation, growth at stressful high temperature (HT; 29°C) is highly mutagenic, increasing the mutation rate to 12 × 10−9.


结果2-突变类型SNV frequency is approximately two- to threefold higher at HT than at ST, and HT-growth causes an ~19- to 23-fold increase in indel frequency, resulting in a disproportionate increase in indels (vs. SNVs).


结果3-indel类型与分布:Most HT-induced indels are 1–2 bp in size and particularly affect homopolymeric or dinucleotide A or T stretch regions of the genome. HT-induced indels occur disproportionately in nucleosome-free regions, suggesting that much HT-induced mutational damage occurs during cell-cycle phases when genomic DNA is packaged into nucleosomes.


结论:We conclude that stressful experimental temperature increases accelerate plant mutation rates and particularly accelerate the rate of indel mutation.


意义:Increasing environmental temperatures are thus likely to have significant mutagenic consequences for plants growing in the wild and may, in particular, add detrimentally to mutational load.


 摘  要 

摘  要

突变同时是遗传多样性与突变负荷的来源。但是,环境温度的增高对于植物突变速率的影响以及对于某种特定突变类型,比如插入/删除(Indel)与单核苷酸变异(SNV)等的影响还不清楚。目前,我们对于由于人类导致的全球温度升高对整个生态系统的影响了解不多,显得相关领域的研究十分重要。本文中,作者通过对29°C下生长11世代的拟南芥进行全基因组范围的突变积累鉴定,研究了温度升高对于拟南芥突变的影响。生长在常温(23°C)条件下的拟南芥每个位点每代的碱基突变率约为7 × 10−9,而生长在高温(29°C)条件下的拟南芥对应数值升高到了12 × 10−9。高温条件下的SNV频率大概是常温条件下拟南芥的两到三倍,另外高温生长下的indel频率是常温生长的19到23倍,这大大超出了SNV频率的增加量。大多由高温诱导的indels是1-2bp的大小,尤其是基因组上同聚或二核苷酸A或T序列区。高温诱导的indels显著富集于无核小体区域,这表明高温诱导的损伤主要发生于细胞周期阶段,该阶段的基因组DNA会被包装进核小体。作者的结论是实验条件下,胁迫温度的升高加速了植物的突变速率,特别是加速了indel的突变速率。因此,不断升高的环境温度可能会对野生植物产生显著的诱变后果,特别是可能增加有害的突变负荷。


 通讯作者 

**Nicholas P. Harberd**


研究方向:

1. 突变与演化;

2. 面向21世纪“超级绿色革命”作物品种的分子育种;

3. 植物对于盐胁迫的响应。


doi: http://www.genome.org/cgi/doi/10.1101/gr.259853.119


Journal: Genome Research

Published date: Dec 17, 2020




http://blog.sciencenet.cn/blog-3158122-1264859.html

上一篇:Plant Communications:植物胚胎发生综述~从模式植物拟南芥到作物
下一篇:the plant journal:光敏色素B抑制由黑暗诱导的下胚轴形成不定根

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