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PNAS:植物SHR-SCR蛋白复合物在根干细胞分裂过程中发挥重要调控作用

已有 4485 次阅读 2020-7-4 08:41 |个人分类:每日摘要|系统分类:论文交流

Protein complex stoichiometry and expression dynamics of transcription factors modulate stem cell division

第一作者Natalie M. Clark

第一单位北卡罗来纳州立大学

通讯作者Rosangela Sozzani


 Abstract 


背景回顾Stem cells divide and differentiate to form all of the specialized cell types in a multicellular organism. In the Arabidopsis root, stem cells are maintained in an undifferentiated state by a less mitotically active population of cells called the quiescent center (QC).


提出问题:Determining how the QC regulates the surrounding stem cell initials, or what makes the QC fundamentally different from the actively dividing initials, is important for understanding how stem cell divisions are maintained.


主要工作:Here we gained insight into the differences between the QC and the cortex endodermis initials (CEI) by studying the mobile transcription factor SHORTROOT (SHR) and its binding partner SCARECROW (SCR).


模型构建:We constructed an ordinary differential equation model of SHR and SCR in the QC and CEI which incorporated the stoichiometry of the SHR-SCR complex as well as upstream transcriptional regulation of SHR and SCR.


预测+试验-复合物水平:Our model prediction, coupled with experimental validation, showed that high levels of the SHR-SCR complex are associated with more CEI division but less QC division.


预测+试验-上游转录调控:Furthermore, our model prediction allowed us to propose the putative upstream SHR regulators SEUSS and WUSCHEL-RELATED HOMEOBOX 5 and to experimentally validate their roles in QC and CEI division.


预测+试验-Timing:In addition, our model established the timing of QC and CEI division and suggests that SHR repression of QC division depends on formation of the SHR homodimer.


结论:Thus, our results support that SHR-SCR protein complex stoichiometry and regulation of SHR transcription modulate the division timing of two different specialized cell types in the root stem cell niche.


 摘  要 


多细胞生物中,干细胞分裂和分化形成所有特化的细胞类型。在拟南芥的根中,干细胞由一群有丝分裂分化不活跃的细胞群,即静止中心(QC)所控制,以维持在一个未分化的状态。至于QC如何调控周边的干细胞起始,或者是什么使得QC与周边活跃分裂的起始细胞在本质上存在不同,这对于理解干细胞是如何被维持的非常重要。本文中,作者通过研究移动转录因子SHR及其结合伴侣SCR,深入了解了QC与皮层/内皮层起始区(CEI)之间的差异。作者构建了一个SHR和SCR在QC与CEI中的常微分方程模型,其中综合了SHR-SCR复合物的化学计量以及SHR与SCR上游的转录调控。作者的模型预测与试验验证均显示高水平的SHR-SCR复合物与更多的CEI分裂、更少的QC分裂有关。此外,该模型预测帮助作者推测出SHR上游调控因子SEUSS和WOX5,并进一步通过试验验证了它们在QC和CEI分裂中所发挥的作用。另外,作者的模型建立了QC和CEI分裂的时间线,并且显示SHR对于QC分裂的抑制依赖于SHR同源二聚体的形成。因此,本文的研究揭示了SHR-SCR蛋白复合物的计量水平以及SHR的转录调控对于根干细胞龛中两类完全不同类型特化细胞的分裂时间控制至关重要。


doi: 10.1073/pnas.2002166117


Journal: PNAS

Published date: June 30, 2020



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