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PNAS:创伤诱导的生长素积累和ERF115表达协同作用于干细胞再生

已有 425 次阅读 2020-7-2 09:13 |个人分类:每日摘要|系统分类:论文交流

Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration

第一作者Balkan Canher

第一单位根特大学

通讯作者Lieven De Veylder


 Abstract 


背景1-植物再生:Plants are known for their outstanding capacity to recover from various wounds and injuries.


提出问题:However, it remains largely unknown how plants sense diverse forms of injury and canalize existing developmental processes into the execution of a correct regenerative response.


背景2-生长素Auxin, a cardinal plant hormone with morphogen-like properties, has been previously implicated in the recovery from diverse types of wounding and organ loss.


研究:Here, through a combination of cellular imaging and in silico modeling, we demonstrate that vascular stem cell death obstructs the polar auxin flux, much alike rocks in a stream, and causes it to accumulate in the endodermis. This in turn grants the endodermal cells the capacity to undergo periclinal cell division to repopulate the vascular stem cell pool. Replenishment of the vasculature by the endodermis depends on the transcription factor ERF115, a wound-inducible regulator of stem cell division. Although not the primary inducer, auxin is required to maintain ERF115 expression. Conversely, ERF115 sensitizes cells to auxin by activating ARF5/MONOPTEROS, an auxin-responsive transcription factor involved in the global auxin response, tissue patterning, and organ formation.


结论:Together, the wound-induced auxin accumulation and ERF115 expression grant the endodermal cells stem cell activity.


意义:Our work provides a mechanistic model for wound-induced stem cell regeneration in which ERF115 acts as a wound-inducible stem cell organizer that interprets wound-induced auxin maxima.


 摘  要 


植物具有非凡的能力,能够从各种创伤中恢复过来。然而,植物如何感知不同的损伤形式以及如何将现有的发育进程调整为符合时宜的再生响应还存在很大的认知空白。生长素,一类有类似于形态发生素(morphogen)特性的基础植物激素,在先前的研究中被认为参与了植物中各种类型的创伤以及器官丢失后的恢复过程。本文中,通过结合细胞成像以及计算建模,作者发现维管干细胞的死亡会阻碍极性的生长素流,就像溪流中的一块岩石一样,导致生长素在内皮层中积累。这反过来赋予了内皮层细胞进行平周细胞分裂的能力,从而能够重新填充维管干细胞池。内皮层细胞对于维管的重新补充依赖于转录因子ERF115,该TF是一个创伤诱导的干细胞分裂调控子。尽管生长素并不是主要的诱导因素,但其对于维持ERF115的表达是必需的。相反,ERF115通过激活ARF5/MP使得细胞对于生长素敏感,ARF5/MP是一个生长素响应转录因子,其参与了植株整体的生长素响应、组织模式建成和器官形成。综上,创伤诱导的生长素积累以及ERF115的表达赋予了内皮层细胞以干细胞活性。本文的研究为创伤诱导的干细胞再生提供了一个机理模型,其中ERF115作为一个创伤诱导的干细胞组织者,负责诠释创伤诱导的生长素极大值点。


 通讯作者 


**Lieven De Veylder**


研究方向:

细胞周期控制基因如何驱动植物细胞增殖。


doi: 10.1073/pnas.2006620117


Journal: PNAS

Published date: June 29, 2020


p.s. 往期相关研究链接:



http://blog.sciencenet.cn/blog-3158122-1240288.html

上一篇:Nature Plants:钙离子信号编码机制研究综述
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