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PNAS:茄科植物单性结实基因Pad-1的功能鉴定

已有 826 次阅读 2020-5-29 08:30 |个人分类:每日摘要|系统分类:论文交流

Loss of function of the Pad-1 aminotransferase gene, which is involved in auxin homeostasis, induces parthenocarpy in Solanaceae plants

第一作者Satoshi Matsuo

第一单位日本国家农业食品研究组织

通讯作者Satoshi Matsuo


 Abstract 


研究背景与问题Fruit development normally occurs after pollination and fertilization; however, in parthenocarpic plants, the ovary grows into the fruit without pollination and/or fertilization.


研究基础Parthenocarpy has been recognized as a highly attractive agronomic trait because it could stabilize fruit yield under unfavorable environmental conditions.


面临困难:Although natural parthenocarpic varieties are useful for breeding Solanaceae plants, their use has been limited, and little is known about their molecular and biochemical mechanisms.


发现基因:Here, we report a parthenocarpic eggplant mutant, pad-1, which accumulates high levels of auxin in the ovaries.


克隆基因:Map-based cloning showed that the wild-type (WT) Pad-1 gene encoded an aminotransferase with similarity to Arabidopsis VAS1 gene, which is involved in auxin homeostasis.


功能鉴定:Recombinant Pad-1 protein catalyzed the conversion of indole-3-pyruvic acid (IPyA) to tryptophan (Trp), which is a reverse reaction of auxin biosynthetic enzymes, tryptophan aminotransferases (TAA1/TARs).


表达模式:The RNA level of Pad-1 gene increased during ovary development and reached its highest level at anthesis stage in WT.


生物学功能:This suggests that the role of Pad-1 in WT unpollinated ovary is to prevent overaccumulation of IAA resulting in precocious fruit-set.


近缘物种:Furthermore, suppression of the orthologous genes of Pad-1 induced parthenocarpic fruit development in tomato and pepper plants.


结论:Our results demonstrated that the use of pad-1 genes would be powerful tools to improve fruit production of Solanaceae plants.


 摘  要 


果实的发育通常发生于授粉和受精之后,然而,在单性结实的植物中,子房能够不经过授粉或受精就发育形成果实。单性结实是一个非常有价值的农艺性状,其可以在不利环境条件下稳定果实产量。尽管,在茄科植物育种过程中,自然的单性结实品种能够有利于育种,但是其受用范围仍然限制,而且目前对于茄科植物单性结实的分子和生化机制并不清楚。本文中,作者报道了一个单性结实茄子突变体pad-1,其会在子房中积累较高水平的生长素。图位克隆显示野生型的Pad-1基因编码一个氨基转移酶,与拟南芥的VAS1基因属于同源基因,该基因在拟南芥中参与生长素的内稳态。重组Pad-1蛋白能够催化吲哚-3-丙酮酸形成色氨酸,是生长素生物合成的一个逆反应酶,即色氨酸氨基转移酶。野生型中,Pad-1基因的RNA水平在子房发育过程中增加,并且在花期达到最高水平。这说明,Pad-1基因在野生型未授粉的子房中,主要是作用于防止IAA的过量积累,从而阻止植株过早座果。此外,抑制番茄和胡椒中的Pad-1直系同源基因能够诱导单性结实的果实发育。本文的结果揭示了利用pad-1基因突变是一个用于茄科植物提升果实产量的有力工具。



doi: 10.1073/pnas.2001211117


Journal: PNAS

Published date: May 27, 2020


p.s. 往期植物单性结实相关研究:




http://blog.sciencenet.cn/blog-3158122-1235435.html

上一篇:The EMBO Journal:低温响应调控子CBF1能够在环境温度下促进拟南芥下胚轴生长
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