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Nature Plants:拟南芥PDK1调控生长素转运和维管发育

已有 441 次阅读 2020-5-14 08:38 |个人分类:每日摘要|系统分类:论文交流

PDK1 regulates auxin transport and Arabidopsis vascular development through AGC1 kinase PAX

第一作者Yao Xiao

第一单位德国慕尼黑工业大学

通讯作者Remko Offringa


 Abstract 


背景回顾:The 3-phosphoinositide-dependent protein kinase 1 (PDK1) is a conserved master regulator of AGC kinases in eukaryotic organisms.


提出问题pdk1 loss of function causes a lethal phenotype in animals and yeasts, but only mild phenotypic defects in Arabidopsis thaliana (Arabidopsis).


主要结果:The Arabidopsis genome contains two PDK1-encoding genes, PDK1 and PDK2. Here, we used clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) to generate true loss-of-function pdk1 alleles, which, when combined with pdk2 alleles, showed severe developmental defects including fused cotyledons, a short primary root, dwarf stature and defects in male fertility. We obtained evidence that PDK1 is responsible for AGC1 kinase PROTEIN KINASE ASSOCIATED WITH BRX (PAX) activation by phosphorylation during vascular development, and that the PDK1 phospholipid-binding Pleckstrin Homology domain is not required for this process.


结论:Our data indicate that PDK1 regulates polar auxin transport by activating AGC1 clade kinases, resulting in PIN phosphorylation.

image.png

Fig 7f, Only unbound PAX can be activated by PDK1S0 or PDK1, resulting in limited auxin efflux. As cellular auxin levels rise, BRX degradation and subcellular trafficking to the nucleus release PAX28, thereby facilitating activation of PAX by PDK1 and the subsequent promotion of auxin efflux by PAX-mediated PIN phosphorylation. g, In addition, auxin activates PIP5K1 expression and stimulates the concentration of PI(4,5)P2 (phospholipid in red) in the PM, resulting in enhanced PDK1 kinase activity.


 摘  要 


3-磷酸肌醇依赖性蛋白激酶1(PDK1)是真核生物中AGC激酶一个保守的主要调控子。pdk1功能缺失突变体会导致动物和酵母致死缺陷,但是在拟南芥中仅会引起轻微的表型缺陷。拟南芥基因组包含两个PDK1编码基因,分别是PDK1PDK2。本文中,作者通过CRISPR-Cas9获得了一个纯合的pdk1等位基因突变体,当与pdk2等位基因突变结合一起时,会引起严重的发育缺陷,包括子叶融合、主根变短、植株变矮以及雄性育性缺陷。进一步的研究显示PDK1通过磷酸化作用于维管发育过程中AGC1激酶PAX的激活,并且PDK1磷脂结合的Pleckstrin同源结构域对于该过程并不重要。本文的研究显示了PDK1通过AGC1激酶磷酸化PIN蛋白,从而调控生长素的极性转运。


 通讯作者 


**Remko Offringa**


研究方向:植物激素生长素在植物发育对于外界环境信号响应中的作用

以及在植物发育状态转变中的作用



doi: 10.1038/s41477-020-0650-2


Journal: Nature Plants

Published date: May 11, 2020



http://blog.sciencenet.cn/blog-3158122-1233079.html

上一篇:Nature Plants:拟南芥中脂质信号通过磷酸化级联调控PIN介导的生长素流
下一篇:Developmental Cell:拟南芥中PLT/Auxin/ARR-B网络控制根分区

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