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First author: Arjun Khakhar; Affiliations: University of Minnesota (明尼苏达大学): Minneapolis, USA
Corresponding author: Daniel F Voytas
Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for future construction of programmable auto-luminescent plant traits, such as light driven plant-pollinator interactions or light emitting plant-based sensors.
植物体内自发生物发光的化学反应
生物发光(bioluminescence)是科学家广泛用于动植物基因表达报告的有力生物学信号。然而,由于需要向这些报告系统提供底物,因而存在某些不可避免的缺陷,比如说成本太高,而且组织侵透不均匀。本文中,作者通过一个由各零件组成、可组合的工具在植物体内重构了一个真菌的生物发光通路FBP。试验显示该系统可以在多个植物、不同组织中均能够生物发光,并且不需要额外添加底物。作者还通过实例显示了可以将该系统部署到植物中,建立自发生物发光报告,用于基因表达和激素流动检测。同时,作者还给出了一个低成本的基因表达谱呈像平台。这些试验技术可用于搭建可编程的自发生物发光植物性状报告系统,比如说由光驱动的植物-授粉者互作,或是发光植物感受器。
通讯:Daniel F Voytas (https://cbs.umn.edu/contacts/daniel-voytas-phd)
研究方向:植物DNA的精确编辑。
doi: 10.7554/eLife.52786
Journal: eLife
Published date: March 25, 2020
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