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J EXP BOT:水稻OVATE家族蛋白基因赋予植株干旱和冷抗性

已有 1638 次阅读 2017-10-3 08:23 |个人分类:每日摘要|系统分类:论文交流

Rice OVATE family protein 6 regulates plant development and confers resistance to drought and cold stresses


First author:Yamei Ma; Affiliations: South China Botanical Garden, Chinese Academy of Sciences (中国科学院华南植物园): Guangzhou, China

Corresponding author: Jianxiong Li (李建雄)


OVATE family proteins (OFPs) regulate multiple aspects of plant growth and development; however, their roles in responses to abiotic stresses (非生物胁迫) have not yet been studied. Here, we report that rice OsOFP6 regulates growth and development, and alters responses to drought and cold stresses. OsOFP6 is expressed in various tissues and developmental stages. Knock-down of OsOFP6 by RNA interference (RNAi) resulted in semi-dwarf stature (不完全矮秆), altered grain shape, and shorter lateral roots (侧根). OsOFP6 was shown to interfere with auxin in regulating lateral root growth and initiation, as RNAi plants had increased density of lateral roots under IAA (indole-3-acetic acid) treatment. OsOFP6 also affects polar auxin transport, which was demonstrated by the different responses of RNAi and OsOFP6-overexpression (OE) plants to treatment with N-1-naphthylphalamic acid (NPA; 生长素运输抑制剂). Furthermore, under drought conditions OE lines showed slower water loss and less accumulation of H2O2 compared with RNAi plants, which displayed faster water loss and higher H2O2, implying that OsOFP6 may confer both drought avoidance (避旱) and drought tolerance in rice plants. In addition, under cold treatment OE and RNAi plants exhibited lower and higher relative electrical conductivity (REC; 相对电导率), respectively, suggesting an important role of OsOFP6 in response to cold stress. These results provide new insights into the multiple functions of OVATE family proteins.


OVATE家族蛋白调控植物生长发育多个方面,然而其在植物应对非生物胁迫中的作用还没有研究过。本文,作者报道了水稻中OsOFP6调控生长发育,改变水稻对干旱和冷胁迫的响应。OsOFP6基因在多个组织、不同发育时期均有表达。通过RNAi敲除掉OsOFP6导致了不完全矮秆的表型,改变颗粒形状,侧根变短。使用IAA处理水稻植株,OsOFP6会与生长素互作调控侧根的生长与起始,RNAi干扰的植株侧根密度增加。通过生长素运输抑制剂NPA处理的RNAi和OsOFP6过表达植株的不同响应表明OsOFP6还会影响生长素的极性运输。此外,在干旱条件下,相对于RNAi干扰植株,OsOFP6过表达植株的水分丢失变缓、H2O2积累减少,表明OsOFP6可能赋予水稻植株避旱性和抗旱性。另外,在冷处理下,OsOFP6过表达植株和RNAi干扰植株的相对电导率一低一高,显示OsOFP6在冷胁迫响应中的作用。本文的研究结果表明了OVATE家族蛋白的多个功能。


通讯:李建雄 (http://sourcedb.scib.cas.cn/zw/rck/200907/t20090713_2066562.html)



个人简介:1990年,华中农业大学,农学系获植物遗传育种学士学位;1995年,华中农业大学,植物遗传育种硕士学位;1998年,华中农业大学,分子生物学博士学位;1998-2000年,武汉大学,生命科学院;2000-2002年,堪萨斯州立大学,植物病理系,访问科学家;2003-2005年,纽约州立大学石溪分校,生物化学与细胞生物学系,助理研究员;2005-2008年,弗吉尼亚大学,生物系,研究科学家;2009年,中国科学院华南植物园, 研究员。


研究方向:以水稻为材料,研究稻曲病的抗性、miRNA对水稻生长发育的影响及OFP家族基因在水稻生长发育过程及对籽粒形状影响的分子机制。


doi: https://doi.org/10.1093/jxb/erx309


Journal: Journal of Experimental Botany
Published data: September 28, 2017.

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