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New Phytologist:甲基转移酶MET1介导拟南芥的不定芽再生

已有 2009 次阅读 2017-10-2 12:28 |个人分类:每日摘要|系统分类:论文交流

DNA METHYLTRANSFERASE1-mediated shoot regeneration is regulated by cytokinin-induced cell cycle in Arabidopsis


First author:Hui Liu; Affiliations: Shandong Agricultural University (山东农业大学): Shandong, China

Corresponding author: Xian Sheng Zhang (张宪省)


  • DNA methylation plays a critical role in diverse biological processes of plants. Arabidopsis DNA METHYLTRANSFERASE1 (MET1; 甲基转移酶) represses shoot regeneration by inhibiting WUSCHEL (WUS) expression, which is essential for shoot initiation. However, the upstream signals regulating MET1 expression during this process are unclear.

  • We analyzed the signals regulating MET1 expression using a number of established strategies, such as genetic analysis, confocal microscopy (共聚焦显微镜), quantitative real-time PCR and chromatin immunoprecipitation (染色质免疫沉淀).

  • MET1 expression patterns underwent dynamic changes with the initiation of WUS during shoot regeneration. The cell cycle regulator E2FA was characterized as an upstream factor directly promoting MET1 expression. Moreover, cytokinin (细胞分裂素) promoted MET1 expression partially by enhancing CYCD3 expression.

  • Our findings reveal that MET1-mediated shoot regeneration is regulated by the cytokinin-induced cell cycle, and provide new insights into the regulation of DNA methylation in shoot regeneration.


DNA甲基化在植物多种生物过程中扮演着重要作用。拟南芥的DNA甲基转移酶MET1会通过抑制对于芽起始有必要作用的WUS基因的表达从而抑制不定芽再生。然而,该过程调控MET1表达的上游还未知晓。

本文作者通过遗传分析、共聚焦显微镜、实时定量PCR及染色质免疫沉淀等方法分析了调控MET1表达的信号通路。
MET1表达模式在不定芽再生过程中WUS基因的起始时处于高度动态的过程中。细胞周期调控子E2FA是直接促进MET1表达的上游因子。此外,细胞分裂素会通过增强CYCD3基因的表达来促进MET1的表达。
本文的研究发现了MET1介导的不定芽再生是通过细胞分裂素诱导的细胞周期所调控的,并且为DNA甲基化调控不定芽再生提供了新的视野。


通讯:张宪省 (http://life.sdau.edu.cn/s/46/t/2027/f6/2c/info63020.htm)


个人简介:张宪省,博士、教授、博士生导师,山东农业大学校长,作物生物学国家重点实验室主任。担任中国植物学会植物细胞生物学专业委员会副主任,中国遗传学会理事,山东省遗传学会理事长,山东省植物学会副理事长,美国植物生物学会会员。《Plant Cell Reports》副主编,《植物学报》和《遗传》编委。


研究方向:1.    植物发育的分子基础: 本研究方向主要研究植物开花时间、有性生殖、种子发育等植物发育的重要科学问题,尤其是研究植物细胞全能性和器官发育的分子机制。目的旨在解析上述发育过程的基因调控网络,阐明植物发育的细胞分子基础;2.    植物生物技术: 本研究方向主要分离控制重要农艺性状的基因,改良小麦和玉米的重要农艺性状和品质性状,提高作物产量,改良作物品质。


doi: 10.1111/nph.14814


Journal: New Phytologist
First Published data: September 27, 2017.

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