文章来自美国匹兹堡大学，发表在《移植国际》杂志上。美国匹兹堡大学Nakao课题组主要致力于氢气对器官移植损伤保护作用的研究，先后发表氢气对小肠、心脏、肺、肾脏、血管等器官移植后损伤方面的高水平研究论文。上周刚发表在肺组织移植和基因表达相互关系的研究。这周又发表氢气水对心脏移植保护作用的研究。

文章中使用的氢气水有两种，一是用金属镁和水反应产生，另一种是用氢气在水中充气泡产生，

充气泡产生氢气溶液是气体溶液研究中最经典传统的手段，过去氢气生物学领域一直认为，这样的方法可能不能制备出符合浓度要求的氢气溶液，但最近有一些研究先后发现，这样的土方法，仍可以制备出具有治疗作用的氢气溶液，这也给普通学者甚至普通的消费者提供了一种制备氢气水的“技术”，就是简单地把氢气用管子通入水中吹泡，大概10分钟，就可以制备出一瓶可以对身体有好处的保健水。氢气的制备非常容易，或者可以直接购买氢气发生器使用。当然作为研究手段，这样的方法仍有不准确的嫌疑。不过这也给过去许多氢气的研究提出一种潜在的挑战：氢气的有效浓度或有效剂量到底有多低？

考虑到许多氢气电极的校对方式为这种充气法，但这样的溶液可能没有达到真正的饱和溶液，这样的标准溶液就非常容易产生误差，导致检测的数据偏大。也许过去许多研究中所声称的氢气浓度，甚至在体检测的氢气浓度都存在比较大的水分。

该研究在证明氢气效应后，重点从组织的炎症因子，白细胞介素2和gama干扰素角度观察氢气对抗炎症的作用，促进线粒体氧化磷酸化代谢相关的酶，提高组织细胞ATP水平，说明氢气不仅具有抗炎症的作用，而且具有提高组织能量代谢的能力。体外实验证明氢气可以减少T细胞的增殖。关于T细胞增殖体外研究，在2009年哈佛大学关于大肠细菌产生氢气治疗肝炎的论文也曾经使用过。当然，两个研究希望说明的问题是不同的，因此尽管是重复，仍具有价值，这就是生物学研究。

Transpl Int. 2012 Aug 14. doi: 10.1111/j.1432-2277.2012.01542.x. [Epub ahead of print]

Hydrogen-supplemented drinking water protects cardiac allografts from inflammation-associated deterioration.

Noda K, Tanaka Y, Shigemura N, Kawamura T, Wang Y, Masutani K, Sun X, Toyoda Y, Bermudez CA, Nakao A.

Source

 Department of Cardiothoracic Surgery, Pittsburgh, PA, USA  Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA  Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China  Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA  Department of Emergency and Critical Care Medicine, Hyogo College of Medicine, Hyogo, Japan.

Abstract

Recent evidence suggests that molecular hydrogen has therapeutic value for disease states that involve inflammation. We hypothesized that drinking hydrogen-rich water (HW) daily would protect cardiac and aortic allograft recipients from inflammation-associated deterioration. Heterotopic heart transplantation with short-course tacrolimus immunosuppression and orthotopic aortic transplantation were performed in allogeneic rat strains. HW was generated either by bubbling hydrogen gas through tap water (Bu-HW) or via chemical reaction using a magnesium stick [Mg + 2H(2) O → Mg (OH)(2)  + H(2) ] immersed in tap water (Mg-HW). Recipients were given either regular water (RW), Mg-HW, Bu-HW, or Mg-HW that had been subsequently degassed (DW). Graft survival was assessed by daily palpation for a heartbeat. Drinking Mg-HW or Bu-HW was remarkably effective in prolonging heart graft survival and reducing intimal hyperplasia in transplanted aortas as compared with grafts treated with RW or DW. Furthermore, T cell proliferation was significantly inhibited in the presence of hydrogen in vitro, accompanied by less production of interleukin-2 and interferon-γ. Hydrogen treatment was also associated with increased graft ATP levels and increased activity of the enzymes in mitochondrial respiratory chain. Drinking HW prolongs survival of cardiac allografts and reduces intimal hyperplasia of aortic allografts.

© 2012 The Authors. Transplant International © 2012 European Society for Organ Transplantation.