氢气抗氧化抗炎症的研究已经有很多，关于氢气治疗一氧化碳中毒的研究，我们过去曾经发表在急救医学杂志上，我们证明氢气对一氧化碳中毒引起的脑组织脱髓鞘改变，其原因可能和氢气的抗氧化抗炎症有关。2年前我曾经到第四军医大学航空临床医学教研室介绍过氢气的研究进展，他们对这一研究也十分有兴趣，于是从那时就开展了相关研究，经过2年多的研究，这个课题组发表了第一篇关于氢气治疗一氧化碳中毒的研究论文。这一研究比较有新意的内容是关于氢气对铁铜和锌离子的影响，另外关于细胞坏死和自噬的形态学研究也属于比较新的研究内容。该论文来自第四军医大学航空临床医学教研室的研究，发表在急救医学杂志上。

氢气作为抗氧化物质可保护脑缺血后自由基引起的氧化损伤，一氧化碳中毒后神经细胞损伤部分原因是自由基增加引起的氧化损伤，本研究的假说为氢气可以保护一氧化碳中毒引起的神经损伤。研究采用氢气生理盐水治疗大鼠一氧化碳中毒。雄性SD大鼠制备成一氧化碳中毒模型，氢气生理盐水每天腹腔注射6 ml/kg。使用检测学习记忆能力的水迷宫和检测动物认知能力的旷场实验。行为学研究结束后，动物被断头处死，采用火焰原子吸收光谱测定法对血清和脑组织中的铜、锌和铁含量进行检测。采用常规的HE和免疫组织化学染色对神经细胞坏死、调亡和自噬进行分析。研究结果发现，氢气生理盐水治疗可以治疗一氧化碳中毒引起的动物认知障碍，减少脑组织神经细胞坏死、调亡和自噬。另外，氢气生理盐水治疗可以减少血清和脑组织中和自由基代谢关系密切的金属离子铁的含量，提高血清中铜的含量。研究结果表明，氢气生理盐水可以有效治疗急性一氧化碳中毒后脑损伤，其治疗机制可能通过改变某些微量元素减少自由基引起氧化损伤有关系。

Effects of Hydrogen-rich Saline on Rats with Acute Carbon Monoxide Poisoning.pdf

Effects of Hydrogen-rich Saline on Rats with Acute Carbon Monoxide Poisoning

Wenlan Wang, PhD∗, Liang Tian, MM∗, Ya Li, MM†, Xiaojun Wang, MM†, Feng Xia, MM∗, Li Li, MM∗, Jinsheng Li, PhD†, Zuoming Zhang, PhD∗,

∗ Department of Clinical Aerospace Medicine, School of Aerospace Medicine, Fourth Military Medical University, Xi' an, Shaanxi Province, China

† Department of Aerospace Hygiene and Health Service, School of Aerospace Medicine, Fourth Military Medical University, Xi' an, Shaanxi Province, China

Received 21 April 2011. Revised 3 August 2011. Accepted 19 January 2012. Available online 13 August 2012.

http://dx.doi.org/10.1016/j.jemermed.2012.01.065, How to Cite or Link Using DOICited by in Scopus (0)Permissions & Reprints

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Abstract

Background

Studies have shown that inhalation of hydrogen gas, which acts as an antioxidant, can protect the brain against free radicals in rats with ischemia-reperfusion. The neuronal damage caused by acute carbon monoxide (CO) poisoning is partly free radical mediated. We hypothesize that hydrogen may prevent neurological damage from CO poisoning.

Objectives

This study is designed to test whether hydrogen (H2)-rich saline will have a protective effect on rats with acute CO poisoning.

Methods

Male Sprague-Dawley rats were subjected to CO poisoning. H2-rich saline was administered by peritoneal injection (6 mL/kg/24 h). We used the Morris water maze and the open field test to determine cognitive function. After cognitive function studies, rats were decapitated and the levels of trace elements copper (Cu), zinc (Zn), and iron (Fe) in serum and brain were assessed by flame atomic absorption spectrometry. Necrosis, apoptosis, and autophagy of neurons were assessed by H-E staining and immunohistochemical staining in another group of rats.

Results

H2-rich saline treatment improved the cognitive deficits and reduced the degree of necrosis, apoptosis, and cell autophagy in rats. Additionally, H2-rich saline decreased the content of Fe in serum and brain in these rats, and increased the content of serum Cu related to free radical metabolism.

Conclusions

H2-rich saline may effectively protect the brain from injury after acute CO poisoning. The mechanism of this protection may be related to lessening oxidative damage by affecting trace elements in vivo.