GmCCD4 controls carotenoid content in soybeans

第一作者：Jinshan Gao

第一单位：中科院东北地理与农业生态研究所

通讯作者：Xianzhong Feng

 Abstract 

研究目的：To better understand the mechanisms regulating plant carotenoid metabolism in staple crop, we report the map‐based cloning and functional characterization of the Glycine max carotenoid cleavage dioxygenase 4 (GmCCD4) gene, which encodes a carotenoid cleavage dioxygenase enzyme involved in metabolizing carotenoids into volatile β‐ionone.

结果1-突变体表型：Loss of GmCCD4 protein function in four Glycine max increased carotenoid content (gmicc) mutants resulted in yellow flowers due to excessive accumulation of carotenoids in flower petals. The carotenoid contents also increase three times in gmicc1 seeds.

结果2-GWAS分析：A genome‐wide association study indicated that the GmCCD4 locus was one major locus associated with carotenoid content in natural population.

结果3-单倍型：Further analysis indicated that the haplotype‐1 of GmCCD4 gene was positively associated with higher carotenoid levels in soybean cultivars and accumulated more β‐carotene in engineered E. coli with ectopic expression of different GmCCD4 haplotypes.

结论：These observations uncovered that GmCCD4 was a negative regulator of carotenoid content in soybean, and its various haplotypes provide useful resources for future soybean breeding practice.

 通讯作者 

doi: 10.1111/pbi.13506

Journal: Plant Biotechnology Journal

First Published: Nov 01, 2020