博文
- 免疫荧光三标
- 热度 1
- Immunofluorescent Protocol Hearts were fixed in 4% paraformaldehyde overnight at room temperature. The following day, hearts were moved to 50% ethanol and stored for up to one week before paraffin embedding. Paraffin sections (5 μ m) were cut through the entire ventricle. All im ...
- 组织固定
- 组织的固定 (一）固定的概念 应用各种方法使病理标本尽量保持其离体前状态的过程称为固定（fixation）。病理标本（样本）离体后，由于微环境的变化将发生自溶和（或）腐败，使其结构破坏。 固定的目的和机制 是：①使蛋白质凝固，终止或减少分解酶的作用，防止自 ...
