留言板
- [5]李蕾
- 老师您好，我是南京理工大学经济管理学院管理科学与工程专业的一名博士生，最近想做一份用户打标签行为的调研（就是您在发表博文时会让您给博文添加标签），但是我缺少研究数据，恳请您在百忙中抽空帮我填写一份问卷，问卷大约需要您5分钟的时间，非常感谢您！！
问卷地址是：http://www.sojump.com/jq/2709468.aspx 将此链接复制进浏览器的地址栏，然后按回车键即可，非常感谢您！！！
- [4]etwf123
- 尊敬的刘全生老师
您好~
我是负责北方医疗科研实验室的技术服务工程师王飞。
康成生物是专业提供生命科学前沿研究技术的企业，致力于向客户提供创新性产品及技术服务，为客户在生命科学领域进行探索创造有利条件并最终实现 长期价值。 康成生物的产品和技术服务涵盖生命科学研究技术的诸多方面。 主要技术平台包括：生物芯片技术平台（microRNA 芯片（丹麦Exqion公司)，.长链非编码RNA芯片（美国arraystar公司）基因芯片（Roche-NImblegen.Agilent)/蛋白芯片(RayBiotech公司），和 ChIP-chip 等Roche-NImblegen旗下品牌）、和Illumina Genome Analyzer IIx测序系统的新一代高通量测序服务
生命科学研究已经全面进入“组学”时代，例如基因组学、表观基因组学、转录组学和蛋白质组学等。生物芯片和高通量测序技术可以为实验者提供详尽准确的DNA、RNA或protein数据，是生命科学研究的最佳工具。康成生物多年来致力于生命科学前沿研究的技术服务，许多客户的实验成果已经发表在国际知名杂志上。本次讲座将重点介绍康成生物在表观基因组与转录组领域的高通量技术平台，并且结合客户发表的最新文章，解析从“课题设计”、“实验服务”到“数据分析”、“功能实验”的解决方案。
一． 表观遗传学研究的技术平台与研究策略
1. DNA甲基化：MeDIP-chip、MeDIP-seq
2. 组蛋白修饰与转录因子调控：ChIP-chip、ChIP-seq
二． 调控型非编码RNA研究平台的应用及研究进展
1. microRNA：miRNA Array、miRNA-seq
2. Long non-coding RNA：LncRNA Array
三． 表达谱芯片在生物学研究中的应用及数据深入挖掘
1. 结合论文实例解析实验技术思路
2. 表达谱芯片数据的有效使用
四． 特定基因集合在mRNA水平的高效定量检测
1. 实时定量PCR芯片的特点与种类
2. 解析经典文章的应用策略
康成生物发展的原动力是追求技术卓越和创新。康成生命科学实验中心拥有一流的实验设备 , 经验丰富的实验技术人员和突出的研发能力。实验中心通过不断地优化和创新，时刻同国际先进水平的生命科学技术发展保持同步。
康成生物通过技术创新和管理优化倾力为客户提供优质和高效的服务。我们的服务宗旨是提升客户的研发技术质量，降低客户的研发总体成本、缩短客户的研发整体周期，与客户建立长期合作关系。 www.kangchen,com.cn 联系 15510008801 王飞
客户使用康成技术服务发表的SCI文章列表
一、国内客户使用康成Exiqon microRNA芯片技术服务发表的SCI文章
1. Zhou, M., et al., MicroRNA-125b confers the resistance of breast cancer cells to paclitaxel through suppression of pro-apoptotic Bcl-2 antagonist killer 1 (Bak1) expression. J Biol Chem, 2010. 285(28): p. 21496-507.
2. Yi, Z., et al., Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues. J Cancer Res Clin Oncol, 2010. 136(6): p. 855-62.
3. Wu, L., et al., A novel function of microRNA let-7d in regulation of galectin-3 expression in attention deficit hyperactivity disorder rat brain. Brain Pathol, 2010. 20(6): p. 1042-54.
4. Wang, G., et al., Up-regulation of microRNA in bladder tumor tissue is not common. Int Urol Nephrol, 2010. 42(1): p. 95-102.
5. Tie, J., et al., MiR-218 inhibits invasion and metastasis of gastric cancer by targeting the Robo1 receptor. PLoS Genet, 2010. 6(3): p. e1000879.
6. Cheng, J., et al., MicroRNA profiling in mid- and late-gestational fetal skin: implication for scarless wound healing. Tohoku J Exp Med, 2010. 221(3): p. 203-9.
7. Chen, X., et al., MicroRNA145 targets BNIP3 and suppresses prostate cancer progression. Cancer Res, 2010. 70(7): p. 2728-38.
8. Zhang, B., X. Wang, and Y. Wang, Altered gene expression and miRNA expression associated with cancerous IEC-6 cell transformed by MNNG. J Exp Clin Cancer Res, 2009. 28: p. 56.
9. Yan, H.L., et al., Repression of the miR-17-92 cluster by p53 has an important function in hypoxia-induced apoptosis. EMBO J, 2009. 28(18): p. 2719-32.
10. Xu, C.F., C.H. Yu, and Y.M. Li, Regulation of hepatic microRNA expression in response to ischemic preconditioning following ischemia/reperfusion injury in mice. OMICS, 2009. 13(6): p. 513-20.
11. Wang, J., et al., MicroRNA: novel regulators involved in the remodeling and reverse remodeling of the heart. Cardiology, 2009. 113(2): p. 81-8.
12. Lian, J., et al., Altered microRNA expression in patients with non-obstructive azoospermia. Reprod Biol Endocrinol, 2009. 7: p. 13.
13. Ju, X., et al., Differential microRNA expression in childhood B-cell precursor acute lymphoblastic leukemia. Pediatr Hematol Oncol, 2009. 26(1): p. 1-10.
14. Guo, C.J., et al., miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis. J Hepatol, 2009. 50(4): p. 766-78.
15. Guo, C.J., et al., Changes in microRNAs associated with hepatic stellate cell activation status identify signaling pathways. FEBS J, 2009. 276(18): p. 5163-76.
16. Ding, J., et al., Embryonic stem cells derived from somatic cloned and fertilized blastocysts are post-transcriptionally indistinguishable: a MicroRNA and protein profile comparison. Proteomics, 2009. 9(10): p. 2711-21.
17. Huang, Y.S., et al., Microarray analysis of microRNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis. J Gastroenterol Hepatol, 2008. 23(1): p. 87-94.
18. Hu, S.J., et al., MicroRNA expression and regulation in mouse uterus during embryo implantation. J Biol Chem, 2008. 283(34): p. 23473-84.
19. Yang, B., et al., The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2. Nat Med, 2007. 13(4): p. 486-91.
20. Wang, C. and Q. Li, Identification of differentially expressed microRNAs during the development of Chinese murine mammary gland. J Genet Genomics, 2007. 34(11): p. 966-73.
二、国内客户使用康成SABiosciences PCR芯片技术服务发表的SCI文章
1. Zhang, L., et al., Escin attenuates cognitive deficits and hippocampal injury after transient global cerebral ischemia in mice via regulating certain inflammatory genes. Neurochem Int, 2010. 57(2): p. 119-27.
2. Xu, Z.Y., J.S. Chen, and Y.Q. Shu, Gene expression profile towards the prediction of patient survival of gastric cancer. Biomed Pharmacother, 2010. 64(2): p. 133-9.
3. Xu, X.L., et al., The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor. Carcinogenesis, 2010. 31(2): p. 167-74.
4. Shi, W.D., et al., Identification of liver metastasis-related genes in a novel human pancreatic carcinoma cell model by microarray analysis. Cancer Lett, 2009. 283(1): p. 84-91.
5. Gu, H., et al., Effects of chronic mild stress on the development of atherosclerosis and expression of toll-like receptor 4 signaling pathway in adolescent apolipoprotein E knockout mice. J Biomed Biotechnol, 2009. 2009: p. 613879.
6. Duan, H., et al., Effect of burn injury on apoptosis and expression of apoptosis-related genes/proteins in skeletal muscles of rats. Apoptosis, 2009. 14(1): p. 52-65.
三、国内客户使用康成Raybiotech抗体芯片技术服务发表的SCI文章
1. Yin, M., et al., Lack of apoE causes alteration of cytokines expression in young mice liver. Mol Biol Rep, 2010. 37(4): p. 2049-54.
2. Li, H., et al., Dendritic cell-activated cytokine-induced killer cells enhance the anti-tumor effect of chemotherapy on non-small cell lung cancer in patients after surgery. Cytotherapy, 2009. 11(8): p. 1076-83.
3. Jiang, X., et al., Increased inflammatory response both in brain and in periphery in presenilin 1 and presenilin 2 conditional double knock-out mice. J Alzheimers Dis, 2009. 18(3): p. 515-23.
4. Chen, L.L., et al., Evaluation of immune inhibitory cytokine profiles in epithelial ovarian carcinoma. J Obstet Gynaecol Res, 2009. 35(2): p. 212-8.
5. Qin, D., et al., Induction of lytic cycle replication of Kaposi's sarcoma-associated herpesvirus by herpes simplex virus type 1: involvement of IL-10 and IL-4. Cell Microbiol, 2008. 10(3): p. 713-28.
6. Niu, Q.X., et al., Induction of inflammatory cytokine release from human umbilical vein endothelial cells by agonists of proteinase-activated receptor-2. Clin Exp Pharmacol Physiol, 2008. 35(1): p. 89-96.
7. Li, A., et al., Dysfunction of splenic macrophages in cirrhotic patients with hypersplenism and HBV infection. Am J Med Sci, 2008. 336(1): p. 32-8.
8. Kong, X.N., et al., LPS-induced down-regulation of signal regulatory protein {alpha} contributes to innate immune activation in macrophages. J Exp Med, 2007. 204(11): p. 2719-31.
四、国内客户使用康成Springbio抗体芯片技术服务发表的SCI文章
1. Lou, J., et al., Comparison between two kinds of cigarette smoke condensates (CSCs) of the cytogenotoxicity and protein expression in a human B-cell lymphoblastoid cell line using CCK-8 assay, comet assay and protein microarray. Mutat Res, 2010. 697(1-2): p. 55-9.
2. Hao, Y., et al., IPO-38 is identified as a novel serum biomarker of gastric cancer based on clinical proteomics technology. J Proteome Res, 2008. 7(9): p. 3668-77.
五、国内客户使用康成Agilent 表达谱芯片技术服务发表的SCI文章
1. Guo, X., et al., Homeobox gene IRX1 is a tumor suppressor gene in gastric carcinoma. Oncogene, 2010. 29(27): p. 3908-20.
六、国内客户使用康成ChIP/MeDIP技术服务发表的SCI文章
1. Yan, X.J., et al., Exome sequencing identifies somatic mutations of DNA methyltransferase gene DNMT3A in acute monocytic leukemia. Nat Genet, 2011.
2. Liu, B.L., et al., Quantitative detection of multiple gene promoter hypermethylation in tumor tissue, serum, and cerebrospinal fluid predicts prognosis of malignant gliomas. Neuro Oncol, 2010. 12(6): p. 540-8.
3. Zhang, L., et al., Genome-wide analysis of histone H3 lysine 27 trimethylation by ChIP-chip in gastric cancer patients. J Gastroenterol, 2009. 44(4): p. 305-12.
4. Zhang, L., et al., Genome-wide analysis of histone H3 lysine 4 trimethylation in peripheral blood mononuclear cells of minimal change nephrotic syndrome patients. Am J Nephrol, 2009. 30(6): p. 505-13.
5. Hu, J.L., et al., The N-terminus of histone H3 is required for de novo DNA methylation in chromatin. Proc Natl Acad Sci U S A, 2009. 106(52): p. 22187-92.
七、国内客户使用康成Agilent aCGH芯片技术服务发表的SCI文章
1. Xu, X.L., et al., The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor. Carcinogenesis, 2010. 31(2): p. 167-74.
八、国内客户使用康成Nimblegen aCGH芯片技术服务发表的SCI文章
1. Zhang, Y., et al., Array-CGH detection of UGT2B28 gene deletion in a girl with primary amenorrhea and hyperandrogenism. Int J Gynaecol Obstet, 2010. 109(2): p. 164-6.
九、国内客户使用康成Nimblegen 表达谱芯片技术服务发表的SCI文章
1. Li, Z., et al., From the Cover: Feature Article: Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation. Proc Natl Acad Sci U S A, 2011. 108(8): p. 3116-23.
2. Fan, C., et al., Random small interfering RNA library screen identifies siRNAs that induce human erythroleukemia cell differentiation. Leuk Lymphoma, 2011. 52(3): p. 502-14.
3. Zheng B, Han M, Shu YN, et al., HDAC2 phosphorylation-dependent Klf5 deacetylation and RARα acetylation induced by RAR agonist switch the transcription regulatory programs of p21 in VSMCs. Cell Res., 2011; doi:10.1038/cr.2011.34
15510008801 etwf123@126.com
祝好~~~
- [3]吴锁伟
- 呵呵，过奖了！有空来看看，常联系！
- [2]孟祥兵
- 老吴，你的文采不错啊，多联系！
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