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PNAS:拟南芥种子DNA损伤响应促进种子萌发和幼苗生长

已有 1179 次阅读 2022-8-1 11:30 |个人分类:每日摘要|系统分类:论文交流

Seed DNA damage responses promote germination and growth in Arabidopsis thaliana

第一作者Wanda M. Waterworth

第一单位利兹大学

通讯作者Christopher E. West


 Abstract 

背景回顾The desiccated, quiescent state of seeds confers extended survival of the embryonic plant. However, accumulation of striking levels of genome damage in quiescence impairs germination and threatens plant survival.


提出问题The mechanisms by which seeds mitigate this damage remain unclear. 


主要发现Here, we reveal that imbibed Arabidopsis seeds display high resistance to DNA damage, which is lost as seeds advance to germination, coincident with increasing cell cycle activity. 


结果1-种子降低DNA损伤的方法In contrast to seedlings, we show that seeds minimize the impact of DNA damage by reducing meristem disruption and delaying SOG1-dependent programmed cell death. This promotes root growth early postgermination. 


结果2-SOG1途径In response to naturally accumulated DNA damage in aging seeds, SOG1 activates cell death postgermination. SOG1 activities are also important for promoting successful seedling establishment. These distinct cellular responses of seeds and seedlings are reflected by different DNA damage transcriptional profiles. 


结果3-基因组维持途径Comparative analysis of DNA repair mutants identifies roles of the major genome maintenance pathways in germination but that the repair of cytotoxic chromosomal breaks is the most important for seed longevity


结论Collectively, these results indicate that high levels of DNA damage incurred in seeds are countered by low cell cycle activity, cell cycle checkpoints, and DNA repair, promoting successful seedling establishment. 


展望Our findings reveal insight into both the physiological significance of plant DNA damage responses and the mechanisms which maintain seed longevity, important for survival of plant populations in the natural environment and sustainable crop production 


 摘 要 

种子处于干燥和休眠状态,能够延长有胚植物的存活时间。然而,休眠状态下基因组损伤的积累对种子萌发有负面影响,并威胁着植物的生存。但是,种子减轻基因组损害的机制尚不清楚。本文中,作者发现吸胀的拟南芥种子对DNA损伤具有较高的抗性,但是随着种子萌发,这种抗性会逐渐丢失,与细胞周期活性的增加相一致。与幼苗相反,作者发现种子通过减少分生组织扰乱并延迟SOG1依赖型的细胞程序性死亡,从而最小化DNA损伤。这在种子萌发后的早期,促进了根的生长。在种子发育过程中,响应于自然积累的DNA损伤,SOG1激活程序性细胞死亡。SOG1活性对于促进幼苗的成功建立非常重要。不同的DNA损伤转录图谱,反映了种子和幼苗的不同细胞响应。通过对DNA修复突变体的比较分析,作者鉴定了主要基因组维持途径在种子萌发中的作用,但是细胞毒性染色体断裂的修复对种子的寿命最为重要。综上,这些研究结果表明,种子可以通过低水平的细胞周期活性、细胞周期检查点和DNA修复来抵消高水平的DNA损伤,从而促进幼苗的成功建立。本文的研究结果揭示了植物DNA损伤响应的生理意义和维持种子长寿的机制,这对植物种群在自然环境中的生存和气候变化下的可持续作物生产至关重要。


doi: https://doi.org/10.1073/pnas.2202172119


Journal: PNAS

Published date: July 18, 2022


Cite:
Wanda M. Waterworth, Rosalind Latham, Dapeng Wang, Mona Alsharif, Christopher E. West. Seed DNA damage responses promote germination and growth in Arabidopsis thaliana. PNAS, 2022, 119(30): e2202172119. DOI: https://doi.org/10.1073/pnas.2202172119



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