注：这里的“清肠” 是指医学需要的清肠。比如说，为了做肠镜检查。

Effects of bowel preparation on the human gut microbiome and metabolome

https://www.nature.com/articles/s41598-019-40182-9

Large bowel preparation may cause a substantial change in the gut microbiota and metabolites. Here, we included a bowel prep group and a no-procedure control group[,] and evaluated the effects of bowel prep on the stability of the gut microbiome and metabolome as well as on recovery. Gut microbiota and metabolome compositions were analyzed by 16S rRNA sequencing and capillary electrophoresis time-of-flight mass spectrometry, respectively. Analysis of coefficients at the genus and species level and weighted UniFrac distance showed that, compared with controls, microbiota composition was significantly reduced immediately after the prep but not at 14 days after it. For the gut metabolome profiles, correlation coefficients between before and immediately after the prep were significantly lower than those between before and 14 days after prep[,] and were not significantly different compared with those for between-subject differences. Thirty-twometabolites were significantly changed before and immediately after the prep, but these metabolites recovered within 14 days. In conclusion, bowel preparation has a profound effect on the gut microbiome and metabolome, but the overall composition recovers to baseline within 14 days. To properly conduct studies of the human gut microbiome and metabolome, fecal sampling should be avoided immediately after bowel prep.

清肠可能会导致肠道微生物群和代谢物发生重大变化。在这里，我们用一个清肠组和一个对照组，来评估清肠对肠道微生物组和代谢组稳定性以及清肠后恢复的影响。我们分别通过 16S rRNA 测序和毛细管电泳飞行时间质谱，分析肠道微生物群和代谢组组成。对属和种水平的系数和加权 UniFrac 距离的分析表明，与对照组相比，微生物群组成在清肠后立即显示出降低，但在之后 14 天没有显著降低。对于肠道代谢组谱，清肠前后的相关系数显示低于清肠前和清肠后 14 天之间的相关系数；并且与两组之间的差异相比没有明显差异。 32 种代谢物在清肠前后发生了显着变化，但这些代谢物在 14 天内就恢复。总之，清肠对肠道微生物组和代谢组有深远的影响，但整体成分在 14 天内恢复到基线。为了正确进行人类肠道微生物组和代谢组的研究，应避免清肠后立即做粪便取样。

评论：

1) I added two commas in the abstract, using brackets.

2) The authors didn't know how to use "as well as" properly.

3) The last sentence is typical "bad" English, BUT most people accept it. Why bad? The action of "to properly conduct studies of the human gut microbiome and metabolome" is carried out by the subject (say, researchers).

Follow me, if you want to improve your writing skill:

如果你想提高科技英语写作能力，请跟我来...

http://blog.sciencenet.cn/blog-306792-1146690.html