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病毒感染细胞的模型,诞生了无数大牛,量产着无数科研成果和科研人员,现在病毒感染线虫的模型终于建立了,在线虫这个无数基因首先被命名被定义的归零地,开展病毒宿主互作的研究潜力是极大的,如果能买股票的话,我立马入手,行情看涨啊~
原始文献:
http://www.ncbi.nlm.nih.gov/pubmed/25078701
P.S.正事儿说完了,发几句牢骚:
为什么只有那个不待见的搜索引擎能找到我想找的David Wang
都是搜索出来的第一条链接:
首先是Bing
然后是百度
最后是某个不待见的搜索引擎
这才是我要找的那个David Wang,不是纽约的fashion photographer,也不是Senior Vice President王啟尚,而是华盛顿大学的教授David Wang。我关注他是因为他在感染线虫的病毒模型的一系列尝试,今天的这篇J Virol算是一个宣告,第一个感染线虫的病毒模型终于建立了!引用“To date, Orsay virus is the first and the only identified virus capable of naturally infecting Caenorhabditis elegans.”
http://www.ncbi.nlm.nih.gov/pubmed/25078701
注意到以前关于线虫 病毒 感染模型的报道
http://www.bbioo.com/experiment/24-87219-1.html
References:
{1} Lu et al. Nature 2005, 436:1040-3 [PMID:16107851].
{2} Wilkins et al. Nature 2005, 436:1044-7 [PMID:16107852].
{3} Schott et al. Proc Natl Acad Sci U S A 2005, 102:18420-4 [PMID:16339901].
以线虫作为病毒感染模型的想法并不新,但这些不是天然感染线虫的病毒。比如我所关心的天然病毒感染 表面细胞 比如 肠道 后,最终会 到哪里去 (比如会不会 最终定居于 神经细胞等 寿命 很长的细胞里),这样的问题,似乎能 在透明的线虫里看到答案。
2014/8/21
病毒晶体结构都出来了~
1Department of BioSciences, Rice University, Houston, TX 77005;
2Graduate Program in Structural and Computational Biology and Molecular Biophysics andVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030; and.
3Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030; and.
4Departments of Molecular Microbiology andPathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
5Department of BioSciences, Rice University, Houston, TX 77005; ytao@rice.edu.
Orsay, the first virus discovered to naturally infect Caenorhabditis elegans or any nematode, has a bipartite, positive-sense RNA genome. Sequence analyses show that Orsay is related to nodaviruses, but molecular characterizations of Orsay reveal several unique features, such as the expression of a capsid-δ fusion protein and the use of an ATG-independent mechanism for translation initiation. Here we report the crystal structure of an Orsay virus-like particle assembled from recombinant capsid protein (CP). Orsay capsid has a T = 3 icosahedral symmetry with 60 trimeric surface spikes. Each CP can be divided into three regions: an N-terminal arm that forms an extended protein interaction network at the capsid interior, an S domain with a jelly-roll, β-barrel fold forming the continuous capsid, and a P domain that forms surface spike projections. The structure of the Orsay S domain is best aligned to T = 3 plant RNA viruses but exhibits substantial differences compared with the insect-infecting alphanodaviruses, which also lack the P domain in their CPs. The Orsay P domain is remotely related to the P1 domain in calicivirus and hepatitis E virus, suggesting a possible evolutionary relationship. Removing the N-terminal arm produced a slightly expanded capsid with fewer nucleic acids packaged, suggesting that the arm is important for capsid stability and genome packaging. Because C. elegans-Orsay serves as a highly tractable model for studying viral pathogenesis, our results should provide a valuable structural framework for further studies of Orsay replication and infection.
crystallography; microscopy; virology
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