安装Primer3后，发现直接使用命令

primer3_core <example >example.out

出现错误：

“PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified”

1. 一种解决办法是输入命令时加入-default_version=1的参数

primer3_core  -default_version=1  -output=example.out  example

2. 另外一种解决办法如下：

查来一下发现Primer3文档中有这样的改变：

“2.5. IMPORTANT: because PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1,PRIMER_THERMODYNAMIC_PARAMETERS_PATH must point to the right location.This tag specifies the path to the directory that contains all theparameter files used by the thermodynamic approach. In Linux, thereare two *default* locations that are tested if this tag is notdefined: ./primer3_config/ and /opt/primer3_config/. For Windows,there is only one default location: .primer3_config.  If the theparameter files are not in one these locations, be sure to setPRIMER_THERMODYNAMIC_PARAMETERS_PATH.”

所以就只好在创建文件夹（需要root权限）

mkdir /opt/primer3_config

然后将primer3-2.3.6/src/primer3_congfig/下的所有文件都移动创建的文件夹中

cp -R ./primer3_congfig/*   /opt/primer3_config

重新使用命令就不会报错了

primer3_core <example >example.out