Capillary electrophoresis of DNA fragments in mixed solution of hydroxyethyl cellulose

Zhenqing Li,   Chenchen Liu,   Yoshinori Yamaguchi,   Yi Ni,   Qingxiang You and   Xiaoming Dou  

Abstract:DNA fragments (0.1-10.0 kbp (kbp, kilo base pairs)) separation by capillary electrophoresis in mixed hydroxyethyl cellulose (HEC) polymer with different molecular weight (Mw) (90 k, 250 k, 720 k and 1300 k) was performed in this work. The effects of important factors, such as the ratio of the mixed polymer composition and the concentration of the mixing polymer, were investigated. Results show that it cannot only shorten the migration time of DNA without great deterioration in resolution, but also can decrease the viscosity of the polymer, and thus make it easy to fill into the capillary. Finally, we separated the φ×174-Hirc II and λ-EcoT14 I DNA digest with high resolution in the mixed HEC solution within 18 min.

http://pubs.rsc.org/EN/content/articlelanding/2014/ay/c3ay41965g/unauth#!divAbstract