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题目:SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis
主要是发现了早期结肠癌的一个生物标记分子,可以帮助我们对于早期的结肠癌进行诊断,得到有效的治疗,以免以后恶化。
路线图:SPG20甲基化-------spartin蛋白表达下降--------胞质分裂停滞
前面的英文句型:
1 here we show that
2the present study show that
3In a previous epigenome study, we identified a list of genes
4.。。gene located in the chromosome band
5 。。。encode 。。。 which contain 。。domain and
6.。。is a multifunction proteins which found
7it hase also been reported that
8 in the present study, the promotor methylation ststuse of the
9 we show that the。。
结果分析
1Identification of SPG20 as a biomarker for colorectal cancer
by using。。。。。we found that
用了芯片筛选到方法,在正常和腺瘤及癌症的细胞中找出甲基化差异额基因启动子,发现了SPG20有差异,还用了数量特异的甲基化PCR检测。
2 Real-time quantitative analysis shows that SPG20 promoter hypermethylation causes loss of gene expression
用了实时反转录PCR来检测不同细胞的mRNA表达量,是第一个实验的延续,还用了表观遗传有关的药物处理
3Hypermethylated colon cancer cell lines do not express the SPG20 protein product Spartin
用了western blotting和免疫荧光检测技术来分析蛋白的表达,结果和对照相比没有蛋白的表达,也与前面的实验相对应。
3 Spartin localizes to the spindle poles and the cytokinesis bridge of dividing daughter cells
in summary ,detection and quantification
this should 被
expored further
we further show that pomoter
with the loss off
our data not only identify a promising biomaker for
but also reveal a cellular mechanism that could be involved in the early
a number of additional roles have been described for ,including int
in the present study ,we observed that even through colon cancer cell lines with convoluted
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