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(一日一记) 文章修改稿中的修改（语法）
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1. to check---->to examine
2. with the PCR based method---->using the PCR-based method
3. investigate, study
4.was missing---->was absent
5. the DNA fragment 593---->DNA fragment 593
6. after cloning and selecting---->after cloing and selection
7. was still not isolated so far---->has not been islated
8. the primer LMW-Full---->primer LMW-Full
9.13 unique genes matched with 12 kinds of clones---->13 unique genes matched to 12 types of clones
10. the other 11 clones each only contained one gene---->each of the other 11 clones contained only one gene
11. detected from the genomic DNA---->obtained from genomic DNA
12. three genes absent in the results of Chinese Spring---->three genes, absent in the experiments with Chinese Spring
13. sequence of the gene corresponding to DNA fragment 593 in Chinese Spring was still not identified and whether it belonged to LMW-GS genes was still unclear----> the gene sequence corresponding to DNA fragment 593 in Chinese Spring remains unidentified and whether it belongs to the LMW-GS gene family remains unclear
14. the other members all contained internal stop codons in their coding regions, which were considered as pseudogenes---->the other members all contained internal stop codons in their coding regions, and so were considered as pseudogenes
15. Each comprised four main structural regions including....----> Each consisted of four main structural regions:...
16. the remaining active genes were all classified into LMW-m-type genes---->the remaining active genes were all classified as LMW-m-type genes
17. ## obviously were the most abundant in bread wheat---->## were clearly the most abundant in bread wheat
18. In addition, there were some special genes---->Additionally, several particular genes
19. was well investigated---->was fully investigated
20. the whole coding sequences---->the entire coding sequence
21. like that successfully used in isolating...---->, as was done successfully to isolate...
22. were usefull to tell the recombinant clones----> were helpful in detecting the recombinant clones
23. was aimless and increased the cost greatly---->was not efficient and greatly increased costs
24. which increased the difficulty in the subsequent sequence analysis----> making subsequent sequence analysis more difficult.
25. to improve the efficiency of the clone screening---->to improve clone screening efficiency
26. Less than 50 clones sequenced were enough to---->fewer than 50 clones sequenced were sufficient to
27. up to now---->to date
28. compositions of the gene family are still ambiguous---->variations of the gene family remain unclear
29. mainly---->primarily
30. powerful---->successful
31. if not considering---->disregarding
32. genes identified from bread wheat varieties with the PCR based method---->genes identified from bread wheat varieties USING the PCR-based method
33. Electrophoresis of the PCR products amplified with the conserved primer in an agarose gel. Full-length genes were amplified with LMW-Full---->Agarose gel electrophoresis of full-length LMW-GS genes amplified using conserved primers LMW-Full
34. 1/3 members of the LMW-GS gene family---->1/3 of the LMW-GS gene family members
34.bread wheat improve programs---->bread wheat improvement programs
35. highest number of ---->largest number of
36. characterizing genes with complete sequences---->characterizing complete gene sequences
37. with the PCR-based method---->using the PCR-based method
38. compared to---->compared with---->in contrast to
39. the genes coding for...---->the genes encoding...
https://blog.sciencenet.cn/blog-508050-422134.html
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