Agrobacterium: From Biology to Biotechnology Edited by Tzvi Tzfira Department of Molecular, Cellular and Developmental Biology University of Michigan Ann Arbor, MI, USA and Vitaly Citovsky Department of Biochemistry and Cell Biology State University of New York Stony Brook, NY, USA
steps in transformation:
1. Agrobacterium attachment to the plant cell;
2. Transfer of T-strands and virulence effector proteins through the plant plasma membrane into the plant cell;
3. T-complex and super-T-complex formation and subsequent cytoplasmic trafficking;
4. Nuclear targeting;
5. Targeting of the super-Tcomplex to chromatin;
6. Removal of proteins from the super-T-complex prior to T-DNA integration;