博文
Argonaute HITS-CLIP decodes microRNA–mRNA interaction maps
|||
Nature 2009.7.23最新一篇文章介绍miRNA靶体识别的文章Argonaute HITS-CLIP decodes microRNA–mRNA interaction maps
目前很多miRNA靶体预测软件可以预测miRNA的多个靶体，但生物信息学分析的假阳性率很高，了解其准确作用位点非常必要。
原始出处：Nature 460, 479-486 (23 July 2009) | doi:10.1038/nature08170
http://www.nature.com/nature/journal/v460/n7254/full/nature08170.html
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein–RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein–RNA complexes in mouse brain. This produced two simultaneous data sets—Ago–miRNA and Ago–mRNA binding sites—that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA–mRNA interactions.
https://blog.sciencenet.cn/blog-290837-247477.html
上一篇：今夜,我是如此的想你!
下一篇：注定要守望一生!
目前很多miRNA靶体预测软件可以预测miRNA的多个靶体，但生物信息学分析的假阳性率很高，了解其准确作用位点非常必要。
原始出处：Nature 460, 479-486 (23 July 2009) | doi:10.1038/nature08170
http://www.nature.com/nature/journal/v460/n7254/full/nature08170.html
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein–RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein–RNA complexes in mouse brain. This produced two simultaneous data sets—Ago–miRNA and Ago–mRNA binding sites—that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA–mRNA interactions.
https://blog.sciencenet.cn/blog-290837-247477.html
上一篇：今夜,我是如此的想你!
下一篇：注定要守望一生!
