中文名：生物素

国际站：Biotin | 生物素

CAS：58-85-5

品牌：MedChemExpress (MCE)

存储条件：Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.

生物活性：生物素（维生素 B7）是一种水溶性 B 族维生素，可作为人体五种羧化酶的辅酶，参与脂肪酸、异亮氨酸和缬氨酸的合成以及糖异生。生物素对于细胞生长、脂肪酸的产生以及脂肪和氨基酸的代谢是必需的^[1][2][3]。

体外：生物素对乳腺癌 (T47D) 细胞的亲和力高于对正常乳腺上皮 (MCF-12A) 细胞的亲和力，K_m 分别为 9.24 μM 和 53.1 μM^[4].生物素（0.09-100 μM；0-70 分钟）被 T47D 细胞以剂量依赖性方式摄取，V_max 为 27.34 pmol/mg 蛋白质/分钟^[4] .生物素（1-1000 nM；24 小时）在恢复 7β-OHC (50 µM) 诱导的细胞死亡和降低细胞粘附方面发挥作用^[5] .

体内：生物素（15 毫克/千克/天；口服；12 天）改善链脲佐菌素诱导的（150 毫克/千克；腹腔注射）糖尿病小鼠的肾毒性^[6]。生物素（0.012 毫克/ kg/d; po; 70 d) 剂量不足对鱼头肾、脾脏和皮肤免疫功能的影响^[7].

参考文献：

[1]. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38.

[2]. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S.[3]. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46.

[4]. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43.

[5]. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561.

[6]. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9.

[7]. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234.