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Wang, Y., Du, F., Wang, J. et al. Improving bread wheat yield through modulating an unselected AP2/ERF gene. Nat. Plants 8, 930–939 (2022). https://doi.org/10.1038/s41477-022-01197-9
Inflorescence architecture determines grain number per spike花序结构决定每穗粒数
用二攵瘫菅橹ば÷笾械幕蚬δ�
自然遗传变异对作物的驯化和育种有很大的贡献1,2。有利的自然变异长期以来一直用于作物选育,是现代知识指导育种的基本资源。在快速和大规模的环境变化下养活不断增长的人口是一个巨大的挑战。创造新的等位基因和识别新的位点将进一步增强性状,甚至产生新的性状3。面包小麦(Triticum aestivum L.)是世界上大部分人口碳水化合物和蛋白质的主要来源,作为一种具有独特进化约束的近交作物,其遗传多样性相对较低4,5。尽管驯化和数十年的选择已经塑造了目前的作物,但人们曾设想,有针对性的基因调整将进一步提高产量3。为了获得额外的有益等位基因,其他温度较高的牧草品种,如大麦(Hordeum vulgare L.)和短柄草(Brachypodium disachyon)已被用于加速面包小麦的遗传解剖6 8。
Results:
1-Isolation and characterization of BdDUO1.
a.短柄草表型 b.TDNA插入,cd为敲除,ef回补
2-Bread wheat DUO-B1 affects spike inflorescence architecture
将短柄草上述基因的同源基因在Bread wheat中找到三个同源基因,根据表达量锁定DUO-B1
we observed that there is no significant difference in the timing of floral transition. DUO-B1不会引起花器官转化时间的性状变化。
Microanatomy observation by scanning electron microscopy showed no difference in inflorescence between wild-type plants and the two mutants during the early stages of spike development19 (Fig. 3g–i).
3-Arabidopsis thaliana ERF12 regulates shoot branching.
上述基因的拟南芥同源基因ERF12功能验证
4-duo-B1 mutations enhance bread wheat yield.
5-DUO-B1 is an unexplored locus in domestication and breeding.
6-DUO-B1 regulates cell division, cytokinin signalling and meristem activity.
7-DUO-B1 promotes BHt /WFZP expression.
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