背景回顾：The WUSCHEL-related homeobox (WOX) transcription factors WOX11 and WOX12 regulate adventitious rooting and responses to stress. 

提出问题：The underlying physiological and molecular regulatory mechanisms in salt stress tolerance remain largely unexplored.

主要研究：Here, we characterized the roles of PagWOX11/12a from 84K poplar (Populus alba × P. glandulosa) and the underlying regulatory mechanism in salt stress.

结果1-表达模式：PagWOX11/12a was strongly induced by salt stress in roots. 

结果2-过表达和抑制表达的表型：Overexpression of PagWOX11/12a in poplar enhanced salt tolerance, as evident by the promotion of growth-related biomass. In contrast, salt-treated PagWOX11/12a dominant repression plants displayed reduced biomass growth. 

结果3-过表达和抑制表达的ROS：Under salt stress conditions, PagWOX11/12a-overexpressed lines showed higher reactive oxygen species (ROS) scavenging capacity and lower accumulation of hydrogen peroxide (H2O2) than non-transgenic 84K plants, whereas the suppressors displayed the opposite phenotype. 

结果4-WOX11/12调控ROS的机制：In addition, PagWOX11/12a directly bound to the promoter region of PagCYP736A12 and regulated PagCYP736A12 expression. The activated PagCYP736A12 could enhance ROS scavenging, thus reducing H2O2 levels in roots under salt stress in PagWOX11/12a-overexpressed poplars. 

结论：The collective results support the important role of PagWOX11/12a in salt acclimation of poplar trees, indicating that PagWOX11/12a enhances salt tolerance through modulation of ROS scavenging by directly regulating PagCYP736A12 expression in poplar.

 摘 要 

WOX转录因子家族的WOX11和WOX12调控不定根的形成和对胁迫的响应。然后，WOX11/12在植物盐耐受性方面的潜在生理和分子调控机制仍不清楚。本文中，作者研究了84K杨中PagWOX11/12a的功能及其在盐胁迫下的调控机制。根中PagWOX11/12a基因的表达量受到盐胁迫的显著诱导。杨树中过表达PagWOX11/12a基因能够增强耐盐性，其能促进盐胁迫下与生长相关的生物量。相反，盐处理的PagWOX11/12a显性抑制株系表现出生物量降低的现象。在盐胁迫的条件下，PagWOX11/12a过表达株系表现出更强的活性氧清除能力，并且相比于非转基因的84K杨，积累的过氧化氢更少，而抑制株系表现出相反的表型。另外，PagWOX11/12a 能够直接结合到PagCYP736A12基因的启动子上，调控PagCYP736A12基因的表达。而激活的PagCYP736A12能够增强植株清除活性氧物质的能力，从而导致PagWOX11/12a过表达株系在盐胁迫下根中积累的过氧化氢水平保持在较低水平。综上，本文的研究结果揭示了PagWOX11/12a在杨树对盐适应中的重要作用，并且解析了PagWOX11/12a通过直接调控PagCYP736A12基因的表达，增强活性氧清除能力，从而促进杨树的耐盐性。

 通讯作者 

** 卢孟柱 **