提出需求：Demands for clade-specific homology inference and analysis have increased in pangenomic era.

软件开发：We proposed a novel method, GeneTribe (https://chenym1.github.io/genetribe/), for homology inference among genetically similar genomes that incorporates gene collinearity and shows better performance than traditional sequence-similarity-based methods in terms of accuracy and scalability.

示例-小麦族数据库：The Triticeae tribe is a typical allopolyploid-rich clade with complex species relationships that includes many important crops such as wheat, barley, and rye. We then built Triticeae-GeneTribe (http://wheat.cau.edu.cn/TGT/) as a homology database, by integrating 12 Triticeae genomes and 3 outgroup model genomes and implemented versatile analysis and visualization functions. 

示例-科学问题1：With macrocollinearity analysis, we were able to construct a refined model illustrating the structural rearrangements of the 4A-5A-7B chromosomes in wheat as two major translocation events.

示例-科学问题2：With collinearity analysis at both the macro- and microscale, we illustrated the complex evolutionary history of homologs of the wheat vernalization gene Vrn2 as a combined result of genome translocation, duplication, and polyploidization and gene loss events.

结论：Our work provides a useful practice for connecting emerging genome assemblies, with awareness of the extensive polyploidy in plants, and will help researchers efficiently exploit genome sequence resources.