||
人的机械化生产流程
基于表观遗传机制的生物生产流水线
需要提供的材料:
一个人的基因组DNA序列,
操作步骤:
一、 初始细胞的制造
1、按照中心法则(三联体密码)生产所需的蛋白质
2、依据基因印迹密码对特定DNA位点进行甲基化修饰,完成基因印迹码的标定
3、依据组蛋白密码对通过1所获取的核心组蛋白进行修饰
4、针对特定的非编码RNA分子进行转录
5、利用分子自组装密码组装细胞器和初始细胞(第一个细胞)
二、 发育工作站的建立
1、 胎儿成型流程软件的编写
2、 自动控制技术及其人体生产装置的设计和制造
三、 胎儿的生产
操作流程
1、 把初始细胞悬浮在无菌孵化液中
2、 启动37度恒温孵育模式
3、 利用自流控控制孵化液中各种营养素、激素水平
4、 测定细胞分裂的速度和质量
5、 当细胞数目达到104时,及时对孵化液内的营养因子和个类激素水平进行实时监控诱导细胞分化
6、 注意观察处于分化期细胞的形貌和基因组的完整性
7、 当胎儿成型之后(细胞数约为1012, 细胞类型约为200种)对各器官的分化质量和功能加以确认(特别注意心跳频率及心电和脑电是否符合规律)
四、 产品下线
1、在确保胎儿各项生理指标正常的情况下,停止营养液、激素的补充
2、利用含氧空气启动胎儿呼吸功能
3、并同时拟合心率和呼吸频率的比率
4、当确认各项指标符合要求时,完全切断营养供应,从孵化器中取出胎儿
The Procedures of making human beings
A Biological production line based on epigenetic mechanism
Material needs to be provided:
A set of human genomic DNA sequence
Operation steps:
I. manufacturing of initial cells
1. Produce the required proteins according to the central dogma (triplet code)
2. Methylation modification of specific DNA sites according to chromatin imprinted code
to complete the calibration of the imprinted code
3. Epigenetically modify the core histones obtained through article 1 according to the histone code
4. Transcription of specific noncoding RNA molecules
5. Assembly of organelles and the initial cells (the first cells) by molecular self-assembly code
II. Establishment of development workstation
1. Self-check of the fetal forming process software, make sure all work
2. Check the Automatic control system of a human body production device, make sure all work
III. Making a fetus
Operation process
1. Suspend the initial cells in sterilized incubation solution
2. Start constant temperature incubation mode at 37 °C
3. Control the levels of various nutrients and hormones in the incubation solution by self-flow control mechanisms
4. Measure the speed and quality of cell division
5. When the number of cells reaches 104, timely monitor the levels of nutrient factors and individual hormones in the incubation solution to induce cell differentiation
6. Pay attention to observe the morphology of the cells in the differentiation stages and the integrity of the genome
7. After the fetus is formed (cell number is around about 1012, the cell types are about 200), check the differentiation quality and function of each organ (pay special attention to whether the heart rate, ECG and EEG conform to the laws)
IV. Product offline
1.Stop the supplements of nutrients and hormones under the condition of ensuring the physiological indexes of fetus are normal
2. Using oxygenated air to activate fetal respiratory function
3. At the same time, the rate between the heart rate and the respiratory rate should match
4. When it is confirmed that the indexes meet the requirements, completely cut off the nutrition supply and take the fetus out of the incubator
当这一天不幸到来的时候...
Archiver|手机版|科学网 ( 京ICP备07017567号-12 )
GMT+8, 2024-9-27 06:28
Powered by ScienceNet.cn
Copyright © 2007- 中国科学报社