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扩增子分析流程

已有 3989 次阅读 2019-4-21 20:08 |个人分类:生物统计软件|系统分类:科研笔记| QIIME

总目录

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QIIME的虚拟机安装

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扩增子分析解读1质控,实验设计,双端序列合并

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扩增子分析解读2提取barcode,质控及样品拆分,切除扩增引物

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扩增子分析解读3格式转换,去冗余,聚类

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扩增子分析解读4去嵌合体,非细菌序列,生成代表性序列和OTU表

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扩增子分析解读5物种注释,OTU表操作

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扩增子分析解读6进化树,Alpha,Beta多样性

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扩增子分析解读7物种分类统计,筛选进化树和其它

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